נגישות
menu      
חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Both Point Mutation and RNA Recombination Contribute to the Sequence Diversity of Citrus Viroid III
Year:
2000
Source of publication :
Virus Genes
Authors :
בר-יוסף, משה
;
.
יאנג, גואנג
;
.
Volume :
20
Co-Authors:

R. A. Owens & R. W. Hammond - Molecular Plant Pathology, Beltsville Agricultural Research Center, Beltsville, MD, 20705, USA

D. Gundersen-Rindal - Insect Biocontrol Laboratories, Beltsville Agricultural Research Center, Beltsville, MD, 20705

T. Candresse - Station de Pathologie Ve´ge´tale, INRA, BP 81, 33883, Villenave d'Ornon Cedex, France

Facilitators :
From page:
243
To page:
252
(
Total pages:
10
)
Abstract:

Field-grown citrus trees often harbor complex mixtures of 4–5 different viroid species, and the presence of citrus viroid III (CVd-III) has been shown to reduce the rate of tree growth without inducing disease. To more fully define the structure of its quasi-species, we have examined nine citrus viroid complexes for the presence of previously undescribed sequence variants of CVd-III. Analysis of 86 full-length cDNAs generated from these nine viroid complexes by RT-PCR revealed the presence of 20 new CVd-III variants. Chain lengths ranged from 293–297 nucleotides, and sequence changes were confined largely to the lower portions of the central conserved region and variable domain. The previously described variants CVd-IIIa (297 nt) and CVd-IIIb (294 nt) were clearly predominant, but phylogenetic analysis indicated that certain isolates may contain representatives of two additional fitness peaks. At least one group of CVd-III variants appears to have arisen as a result of RNA recombination. Populations recovered from diseased/declining trees were the most diverse, but even dwarfing isolates originating from old line Shamouti trees showed considerable variability.

Note:
Related Files :
apscaviroids
phylogenetic analysis
plant diseases and disorders
RNA quasi-species
subviral RNAs
Viroids
viruses and viroids
עוד תגיות
תוכן קשור
More details
DOI :
https://doi.org/10.1023/A:1008144712837
Article number:
0
Affiliations:
Database:
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
49888
Last updated date:
02/03/2022 17:27
Creation date:
13/09/2020 10:34
Scientific Publication
Both Point Mutation and RNA Recombination Contribute to the Sequence Diversity of Citrus Viroid III
20

R. A. Owens & R. W. Hammond - Molecular Plant Pathology, Beltsville Agricultural Research Center, Beltsville, MD, 20705, USA

D. Gundersen-Rindal - Insect Biocontrol Laboratories, Beltsville Agricultural Research Center, Beltsville, MD, 20705

T. Candresse - Station de Pathologie Ve´ge´tale, INRA, BP 81, 33883, Villenave d'Ornon Cedex, France

Both Point Mutation and RNA Recombination Contribute to the Sequence Diversity of Citrus Viroid III

Field-grown citrus trees often harbor complex mixtures of 4–5 different viroid species, and the presence of citrus viroid III (CVd-III) has been shown to reduce the rate of tree growth without inducing disease. To more fully define the structure of its quasi-species, we have examined nine citrus viroid complexes for the presence of previously undescribed sequence variants of CVd-III. Analysis of 86 full-length cDNAs generated from these nine viroid complexes by RT-PCR revealed the presence of 20 new CVd-III variants. Chain lengths ranged from 293–297 nucleotides, and sequence changes were confined largely to the lower portions of the central conserved region and variable domain. The previously described variants CVd-IIIa (297 nt) and CVd-IIIb (294 nt) were clearly predominant, but phylogenetic analysis indicated that certain isolates may contain representatives of two additional fitness peaks. At least one group of CVd-III variants appears to have arisen as a result of RNA recombination. Populations recovered from diseased/declining trees were the most diverse, but even dwarfing isolates originating from old line Shamouti trees showed considerable variability.

Scientific Publication
You may also be interested in