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פותח על ידי קלירמאש פתרונות בע"מ -
Identification of a constitutive 45 kDa soluble protein associated with resistance to downy mildew in muskmelon (Cucumis melo L.), line PI 124111 F
Year:
1992
Authors :
בלס, משה
;
.
בר-יוסף, משה
;
.
Volume :
41
Co-Authors:

Y. Cohen - Department of Life Sciences, Bar-Ilan University, Ramat Gan Israel

Facilitators :
From page:
387
To page:
396
(
Total pages:
10
)
Abstract:

Analysis by sodium dodecylsulphate-polyacrylamide gel electrophoresis of the soluble proteins extracted from muskmelon (Cucumis melo L.), inbred line PI 124111F (PI) with resistance to Pseudoperonospora cubensis (Berk, et Curt.) Rost, and two susceptible cvs, Hemed and AnanasYokneam revealed the presence of a unique 45 kDa protein (P45) in the resistant but not in the susceptible plants. Subcellular fractionation indicated that P45 is a cytoplasmic soluble protein. It was detected in leaves and cotyledons but not in stems or roots. F1 hybrid plants (Hemed × PI) that displayed only a partial resistance against P. cubensis expressed P45 at an intermediate level, whereas plants of the inbred generations F5, F7 and F10 that displayed a high degree of resistance, expressed P45 at a level similar to the parental PI. Back-cross progeny plants [(Hemed × PI) × Hemed] segregate 1:2:1 partially resistant: susceptible: highly susceptible to the disease. The partially resistant plants showed an intermediate level of P45 (similar to F1) whereas the highly susceptible plants had no P45, thus indicating cosegregation of P45 with resistance. The resistance of PI to P. cubensis was found to decrease at a colonization temperature of 12 °C. 35S-methionine in vivo protein labelling revealed a reduction in the intensity of the P45 band in plants incubated for 11 h at 12 °C. The application of P45 in breeding programs of muskmelon for downy mildew resistance and its possible involvement in resistance to P. cubensis are discussed.

Note:
Related Files :
Cucumis melo
disease resistance
Downy mildew
Muskmelon
plant diseases and disorders
plant protection
Resistance
עוד תגיות
תוכן קשור
More details
DOI :
https://doi.org/10.1016/0885-5765(92)90051-V
Article number:
0
Affiliations:
Database:
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
50065
Last updated date:
02/03/2022 17:27
Creation date:
16/09/2020 09:28
Scientific Publication
Identification of a constitutive 45 kDa soluble protein associated with resistance to downy mildew in muskmelon (Cucumis melo L.), line PI 124111 F
41

Y. Cohen - Department of Life Sciences, Bar-Ilan University, Ramat Gan Israel

Identification of a constitutive 45 kDa soluble protein associated with resistance to downy mildew in muskmelon (Cucumis melo L.), line PI 124111 F

Analysis by sodium dodecylsulphate-polyacrylamide gel electrophoresis of the soluble proteins extracted from muskmelon (Cucumis melo L.), inbred line PI 124111F (PI) with resistance to Pseudoperonospora cubensis (Berk, et Curt.) Rost, and two susceptible cvs, Hemed and AnanasYokneam revealed the presence of a unique 45 kDa protein (P45) in the resistant but not in the susceptible plants. Subcellular fractionation indicated that P45 is a cytoplasmic soluble protein. It was detected in leaves and cotyledons but not in stems or roots. F1 hybrid plants (Hemed × PI) that displayed only a partial resistance against P. cubensis expressed P45 at an intermediate level, whereas plants of the inbred generations F5, F7 and F10 that displayed a high degree of resistance, expressed P45 at a level similar to the parental PI. Back-cross progeny plants [(Hemed × PI) × Hemed] segregate 1:2:1 partially resistant: susceptible: highly susceptible to the disease. The partially resistant plants showed an intermediate level of P45 (similar to F1) whereas the highly susceptible plants had no P45, thus indicating cosegregation of P45 with resistance. The resistance of PI to P. cubensis was found to decrease at a colonization temperature of 12 °C. 35S-methionine in vivo protein labelling revealed a reduction in the intensity of the P45 band in plants incubated for 11 h at 12 °C. The application of P45 in breeding programs of muskmelon for downy mildew resistance and its possible involvement in resistance to P. cubensis are discussed.

Scientific Publication
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