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פותח על ידי קלירמאש פתרונות בע"מ -
Advanced molecular tools for breeding in Mediterranean fruit trees: Genome editing approach of Ficus carica L.
Year:
2020
Source of publication :
Acta Horticulturae
Authors :
בוקובזא, שמואל
;
.
כהן, אורית
;
.
כהן-פאר, רעות
;
.
למה, קומאר
;
.
פליישמן, משה
;
.
Volume :
1280
Co-Authors:

Flaishman, M.A. - Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rizon Le-Zion, 7505101, Israel. 
Peer, R.- Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rizon Le-Zion, 7505101, Israel.  
Raz, A. - Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, 76100, Israel.

Cohen, O.- Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rizon Le-Zion, 7505101, Israel. 
 Izhaki, K.- Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rizon Le-Zion, 7505101, Israel. 
Bocobza, S. - Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rizon Le-Zion, 7505101, Israel.
 
Lama, K. - Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rizon Le-Zion, 7505101, Israel. 
 
Pliner, M. - Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, 76100, Israel.

Levy, A. - Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, 76100, Israel. 

Facilitators :
From page:
0
To page:
0
(
Total pages:
1
)
Abstract:

Fruit-tree breeding is a lengthy process with many limitations. Classical breeding strategies using conventional cross-breeding and induced mutations have played an important role in the development of new cultivars in fruit trees. Precise genome editing could be a very useful supplementary tool for the improvement of crop plants. Various genome editing techniques including ZFNs, TALENs, and most recently CRISPR/Cas9 based approaches have been successfully employed for various crop plants including fruit trees. Previously, we used a ZFN-based approach to efficiently induce precise genome editing at specific genomic loci in fig. Furthermore, CRISPR/Cas9-based approaches hold great potential in genome editing due to their simplicity and were efficiently used in the last few years in several fruit trees. Efficient regeneration and transformation systems, a prerequisite for genome editing, were developed in several Ficus carica cultivars. Here we describe efficient CRISPR/Cas9 genome editing in fig. Transgenic fig lines carrying a mutated GUS construct (mGUS), were developed. mGUS editing, using the CRISPR/Cas9 system was confirmed by GUS staining and PCR. Figs are very attractive due to their nutritive and antioxidant properties. Yet, ripened fresh figs are highly perishable and require delicate postharvest handling. Characterizing the transcriptome of ripening fig fruits we found several genes that display altered expression during ripening and related to the ABA and ethylene hormonal processes. Currently we are using the CRISPR/Cas9 methodology to generate non-GM fig fruits that exhibit delayed ripening. The different application of CRISPR/Cas9 methodologies in fruit trees will be discussed. 

Note:
Related Files :
breeding
CRISPR/Cas9
Ficus carica L
Fruit quality
fruit ripening
fruit trees
genome editing
New cultivars
עוד תגיות
תוכן קשור
More details
DOI :
10.17660/ActaHortic.2020.1280.1
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
50349
Last updated date:
02/03/2022 17:27
Creation date:
21/09/2020 17:24
Scientific Publication
Advanced molecular tools for breeding in Mediterranean fruit trees: Genome editing approach of Ficus carica L.
1280

Flaishman, M.A. - Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rizon Le-Zion, 7505101, Israel. 
Peer, R.- Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rizon Le-Zion, 7505101, Israel.  
Raz, A. - Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, 76100, Israel.

Cohen, O.- Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rizon Le-Zion, 7505101, Israel. 
 Izhaki, K.- Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rizon Le-Zion, 7505101, Israel. 
Bocobza, S. - Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rizon Le-Zion, 7505101, Israel.
 
Lama, K. - Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rizon Le-Zion, 7505101, Israel. 
 
Pliner, M. - Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, 76100, Israel.

Levy, A. - Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, 76100, Israel. 

Advanced molecular tools for breeding in Mediterranean fruit trees: Genome editing approach of Ficus carica L.

Fruit-tree breeding is a lengthy process with many limitations. Classical breeding strategies using conventional cross-breeding and induced mutations have played an important role in the development of new cultivars in fruit trees. Precise genome editing could be a very useful supplementary tool for the improvement of crop plants. Various genome editing techniques including ZFNs, TALENs, and most recently CRISPR/Cas9 based approaches have been successfully employed for various crop plants including fruit trees. Previously, we used a ZFN-based approach to efficiently induce precise genome editing at specific genomic loci in fig. Furthermore, CRISPR/Cas9-based approaches hold great potential in genome editing due to their simplicity and were efficiently used in the last few years in several fruit trees. Efficient regeneration and transformation systems, a prerequisite for genome editing, were developed in several Ficus carica cultivars. Here we describe efficient CRISPR/Cas9 genome editing in fig. Transgenic fig lines carrying a mutated GUS construct (mGUS), were developed. mGUS editing, using the CRISPR/Cas9 system was confirmed by GUS staining and PCR. Figs are very attractive due to their nutritive and antioxidant properties. Yet, ripened fresh figs are highly perishable and require delicate postharvest handling. Characterizing the transcriptome of ripening fig fruits we found several genes that display altered expression during ripening and related to the ABA and ethylene hormonal processes. Currently we are using the CRISPR/Cas9 methodology to generate non-GM fig fruits that exhibit delayed ripening. The different application of CRISPR/Cas9 methodologies in fruit trees will be discussed. 

Scientific Publication
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