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פותח על ידי קלירמאש פתרונות בע"מ -
Biochemical Aspects of Fumaric Acid Accumulation by Rhizopus arrhizus
Year:
1986
Authors :
צעדי, אלי
;
.
Volume :
52
Co-Authors:

Kenealy W - Department of Applied Microbiology, The Hebrew University, Jerusalem 91010, Israel ; and E. I. du Pont De Nemours & Co., Central Research and Development Department, Experimental Station, Wilmington, Delaware 19898.

du Preez JC, 

Stieglitz B, 

I Goldberg

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Abstract:

The accumulation and excretion of fumaric acid, and to a lesser extent malic and succinic acids, by Rhizopus arrhizus occurs under aerobic conditions in a high-glucose medium containing a limiting amount of nitrogen and a neutralizing agent (CaCO(3)). An overall four-carbon dicarboxylic acid molar yield of up to 145% (moles of acid produced per mole of glucose utilized) is obtained after incubation for 4 to 5 days. Evidence is presented that fumarate is synthesized from pyruvate via a carboxylation reaction yielding oxaloacetate, which is then converted to malate and further on to fumarate via the reductive reactions of the tricarboxylic acid cycle. The possible formation of fumarate from the normal (oxidative) operation of the tricarboxylic acid cycle was not excluded by the data. Yield, C nuclear magnetic resonance, and enzymatic activity studies were carried out in a strain of R. arrhizus which produces high levels of fumarate from glucose and carbonate. The observed high fumarate molar yield (greater than 100%) can therefore be explained in terms of the carboxylation of pyruvate and the operation of the reductive reactions of the tricarboxylic acid cycle under aerobic conditions.

Note:
Related Files :
Biochemical activity
fumaric acid
Rhizopus arrhizus
עוד תגיות
תוכן קשור
More details
DOI :
10.1128/aem.52.1.128-133.1986
Article number:
0
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Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
50584
Last updated date:
02/03/2022 17:27
Creation date:
29/09/2020 17:43
Scientific Publication
Biochemical Aspects of Fumaric Acid Accumulation by Rhizopus arrhizus
52

Kenealy W - Department of Applied Microbiology, The Hebrew University, Jerusalem 91010, Israel ; and E. I. du Pont De Nemours & Co., Central Research and Development Department, Experimental Station, Wilmington, Delaware 19898.

du Preez JC, 

Stieglitz B, 

I Goldberg

Biochemical Aspects of Fumaric Acid Accumulation by Rhizopus arrhizus

The accumulation and excretion of fumaric acid, and to a lesser extent malic and succinic acids, by Rhizopus arrhizus occurs under aerobic conditions in a high-glucose medium containing a limiting amount of nitrogen and a neutralizing agent (CaCO(3)). An overall four-carbon dicarboxylic acid molar yield of up to 145% (moles of acid produced per mole of glucose utilized) is obtained after incubation for 4 to 5 days. Evidence is presented that fumarate is synthesized from pyruvate via a carboxylation reaction yielding oxaloacetate, which is then converted to malate and further on to fumarate via the reductive reactions of the tricarboxylic acid cycle. The possible formation of fumarate from the normal (oxidative) operation of the tricarboxylic acid cycle was not excluded by the data. Yield, C nuclear magnetic resonance, and enzymatic activity studies were carried out in a strain of R. arrhizus which produces high levels of fumarate from glucose and carbonate. The observed high fumarate molar yield (greater than 100%) can therefore be explained in terms of the carboxylation of pyruvate and the operation of the reductive reactions of the tricarboxylic acid cycle under aerobic conditions.

Scientific Publication
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