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פותח על ידי קלירמאש פתרונות בע"מ -
Expression Kinetics of Regulatory Genes Involved in the Vesicle Trafficking Processes Operating in Tomato Flower Abscission Zone Cells during Pedicel Abscission
Year:
2020
Source of publication :
Life
Authors :
מאיר, שמעון
;
.
סונדארסאן, סריוויגנש
;
.
סלים, שושנה
;
.
פילוסוף-הדס, סוניה
;
.
Volume :
10
Co-Authors:

Srivignesh Sundaresan - Department of Postharvest Science, Agricultural Research Organization (ARO), The Volcani Center, Rishon LeZion 7528809, Israel; The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 7610001, Israel.  

Sonia Philosoph-Hadas - Department of Postharvest Science, Agricultural Research Organization (ARO), The Volcani Center, Rishon LeZion 7528809, Israel


Joseph Riov - The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 7610001, Israel


Shoshana Salim - Department of Postharvest Science, Agricultural Research Organization (ARO), The Volcani Center, Rishon LeZion 7528809, Israel

 
Shimon Meir - Department of Postharvest Science, Agricultural Research Organization (ARO), The Volcani Center, Rishon LeZion 7528809, Israel

Facilitators :
From page:
0
To page:
0
(
Total pages:
1
)
Abstract:

The abscission process occurs in a specific abscission zone (AZ) as a consequence of the middle lamella dissolution, cell wall degradation, and formation of a defense layer. The proteins and metabolites related to these processes are secreted by vesicle trafficking through the plasma membrane to the cell wall and middle lamella of the separating cells in the AZ. We investigated this process, since the regulation of vesicle trafficking in abscission systems is poorly understood. The data obtained describe, for the first time, the kinetics of the upregulated expression of genes encoding the components involved in vesicle trafficking, occurring specifically in the tomato (Solanum lycopersicum) flower AZ (FAZ) during pedicel abscission induced by flower removal. The genes encoding vesicle trafficking components included soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs), SNARE regulators, and small GTPases. Our results clearly show how the processes of protein secretion by vesicle trafficking are regulated, programmed, and orchestrated at the level of gene expression in the FAZ. The data provide evidence for target proteins, which can be further used for affinity purification of plant vesicles in their natural state. Such analyses and dissection of the complex vesicle trafficking networks are essential for further elucidating the mechanism of organ abscission. 

Note:
Related Files :
abscission
flower abscission zone
GTPases
SNARE complex
tomato
transcriptome
Vesicle trafficking
עוד תגיות
תוכן קשור
More details
DOI :
10.3390/life10110273
Article number:
0
Affiliations:
Database:
גוגל סקולר
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
51678
Last updated date:
02/03/2022 17:27
Creation date:
15/11/2020 13:12
You may also be interested in
Scientific Publication
Expression Kinetics of Regulatory Genes Involved in the Vesicle Trafficking Processes Operating in Tomato Flower Abscission Zone Cells during Pedicel Abscission
10

Srivignesh Sundaresan - Department of Postharvest Science, Agricultural Research Organization (ARO), The Volcani Center, Rishon LeZion 7528809, Israel; The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 7610001, Israel.  

Sonia Philosoph-Hadas - Department of Postharvest Science, Agricultural Research Organization (ARO), The Volcani Center, Rishon LeZion 7528809, Israel


Joseph Riov - The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 7610001, Israel


Shoshana Salim - Department of Postharvest Science, Agricultural Research Organization (ARO), The Volcani Center, Rishon LeZion 7528809, Israel

 
Shimon Meir - Department of Postharvest Science, Agricultural Research Organization (ARO), The Volcani Center, Rishon LeZion 7528809, Israel

Expression Kinetics of Regulatory Genes Involved in the Vesicle Trafficking Processes Operating in Tomato Flower Abscission Zone Cells during Pedicel Abscission

The abscission process occurs in a specific abscission zone (AZ) as a consequence of the middle lamella dissolution, cell wall degradation, and formation of a defense layer. The proteins and metabolites related to these processes are secreted by vesicle trafficking through the plasma membrane to the cell wall and middle lamella of the separating cells in the AZ. We investigated this process, since the regulation of vesicle trafficking in abscission systems is poorly understood. The data obtained describe, for the first time, the kinetics of the upregulated expression of genes encoding the components involved in vesicle trafficking, occurring specifically in the tomato (Solanum lycopersicum) flower AZ (FAZ) during pedicel abscission induced by flower removal. The genes encoding vesicle trafficking components included soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs), SNARE regulators, and small GTPases. Our results clearly show how the processes of protein secretion by vesicle trafficking are regulated, programmed, and orchestrated at the level of gene expression in the FAZ. The data provide evidence for target proteins, which can be further used for affinity purification of plant vesicles in their natural state. Such analyses and dissection of the complex vesicle trafficking networks are essential for further elucidating the mechanism of organ abscission. 

Scientific Publication
You may also be interested in