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A. Shirak - Institute of Animal Science, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel .
E. Seroussi - Institute of Animal Science, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel.
N. Zilberman - Institute of Animal Science, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel. 
R. Domokhovsky - Institute of Animal Science, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel.
A. Cnaani - Hubbard Center for Genome Studies, University of New Hampshire, Durham, NH 03824, USA.
D. Kocher - Hubbard Center for Genome Studies, University of New Hampshire, Durham, NH 03824, USA.
M. Ron - Institute of Animal Science, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel.
G. Hulata - Institute of Animal Science, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel.

Expression dynamics of gonad differentiation regulators is similar between zebrafish and mammals. This recent observation prompts us to study candidates for the role of master key regulators (MKRs) in tilapias. We generated genetic markers in 11 putative genes. We genotyped these markers in two purebred species – Oreochromis aureus and O. niloticus – and mapped them to the tilapia linkage groups using genotypic data of 90 individuals of the F2 mapping family. The mapping position of Dax1 connected LG16 and LG21, separated until now, into a single linkage group. The mapping positions of two other candidates overlapped with the mapping positions of two previously reported QTL regions for sex determination. The rest of the genes were mapped to linkage groups 1, 12, 14, and 17.

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Mapping of candidate genes for sex determination in tilapias

A. Shirak - Institute of Animal Science, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel .
E. Seroussi - Institute of Animal Science, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel.
N. Zilberman - Institute of Animal Science, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel. 
R. Domokhovsky - Institute of Animal Science, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel.
A. Cnaani - Hubbard Center for Genome Studies, University of New Hampshire, Durham, NH 03824, USA.
D. Kocher - Hubbard Center for Genome Studies, University of New Hampshire, Durham, NH 03824, USA.
M. Ron - Institute of Animal Science, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel.
G. Hulata - Institute of Animal Science, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel.

Mapping of candidate genes for sex determination in tilapias

Expression dynamics of gonad differentiation regulators is similar between zebrafish and mammals. This recent observation prompts us to study candidates for the role of master key regulators (MKRs) in tilapias. We generated genetic markers in 11 putative genes. We genotyped these markers in two purebred species – Oreochromis aureus and O. niloticus – and mapped them to the tilapia linkage groups using genotypic data of 90 individuals of the F2 mapping family. The mapping position of Dax1 connected LG16 and LG21, separated until now, into a single linkage group. The mapping positions of two other candidates overlapped with the mapping positions of two previously reported QTL regions for sex determination. The rest of the genes were mapped to linkage groups 1, 12, 14, and 17.

Scientific Publication
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