תפריט נגישות
ניגודיות עדינהניגודיות גבוהה
מונוכרום
הדגשת קישורים
חסימת אנימציה
פונט קריא
סגור
איפוס הגדרות נגישות
להורדת מודול נגישות חינם תפריט נגישותניגודיות עדינהניגודיות גבוההמונוכרוםהדגשת קישוריםחסימת אנימציהפונט קריאסגוראיפוס הגדרות נגישותלהורדת מודול נגישות חינםO Hadaya _ Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, 7610001, Rehovot, Israel ; Department of Natural Resources, Institute of Plant Sciences, Agricultural Research Organization - the Volcani Center, 7505101, Rishon LeZion, Israel.
R Bransi-Nicola - The Institute of Applied Research (Affiliated With University of Haifa), The Galilee Society, 20200, Shefa-Amr, Israel.
Y Shalev - Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, 7610001, Rehovot, Israel.,
H Azaizeh _ The Institute of Applied Research (Affiliated With University of Haifa), The Galilee Society, 20200, Shefa-Amr, Israel.
Z Roth - Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, 7610001, Rehovot, Israel.
H Muklada _ Department of Natural Resources, Institute of Plant Sciences, Agricultural Research Organization - the Volcani Center, 7505101, Rishon LeZion, Israel.
T Deutch _Department of Natural Resources, Institute of Plant Sciences, Agricultural Research Organization - the Volcani Center, 7505101, Rishon LeZion, Israel.
S Y Landau _ Department of Natural Resources, Institute of Plant Sciences, Agricultural Research Organization - the Volcani Center, 7505101, Rishon LeZion, Israel.
N Argov-Argaman _ Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, 7610001, Rehovot, Israel.
We assessed the potential of phenolic compounds from Pistacia lentiscus (lentisk) to enhance production of milk constituents in bovine mammary epithelial cells (MEC). MEC were exposed to 0 (control), 1 or 10 ppm of polyphenols from lentisk ethanolic extract (PLEE) for 24 h. PLEE were absorbed by the MEC plasma membrane, but also penetrated the cell to accumulate in and around the nucleus. PLEE increased triglyceride content in the cell and its secretion to the medium, and significantly increased intracellular lipid droplet diameter. Compared to control, PLEE increased dose-dependently the lactose synthesis, secretion of whey proteins, and contents of casein. To evaluate mitochondrial activity under pro-oxidant load, MEC were preincubated with PLEE and exposed for 2 h to H2O2. Exposure to H2O2 increased the proportion of cells with impaired mitochondrial membrane potential twofold in controls, but not in PLEE-pre-treated cells. Accordingly, proton leakage was markedly decreased by PLEE, and coupling efficiency between the respiratory chain and ATP production was significantly enhanced. Thus, lentisk polyphenols divert energy to production of milk fat, protein and lactose, with less energy directed to cellular damage control; alternatively, PLEE enables MEC to maintain energy and oxidative status under extreme metabolic rate required for milk production and secretion, and reduces the limitation on energy required to support production.
O Hadaya _ Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, 7610001, Rehovot, Israel ; Department of Natural Resources, Institute of Plant Sciences, Agricultural Research Organization - the Volcani Center, 7505101, Rishon LeZion, Israel.
R Bransi-Nicola - The Institute of Applied Research (Affiliated With University of Haifa), The Galilee Society, 20200, Shefa-Amr, Israel.
Y Shalev - Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, 7610001, Rehovot, Israel.,
H Azaizeh _ The Institute of Applied Research (Affiliated With University of Haifa), The Galilee Society, 20200, Shefa-Amr, Israel.
Z Roth - Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, 7610001, Rehovot, Israel.
H Muklada _ Department of Natural Resources, Institute of Plant Sciences, Agricultural Research Organization - the Volcani Center, 7505101, Rishon LeZion, Israel.
T Deutch _Department of Natural Resources, Institute of Plant Sciences, Agricultural Research Organization - the Volcani Center, 7505101, Rishon LeZion, Israel.
S Y Landau _ Department of Natural Resources, Institute of Plant Sciences, Agricultural Research Organization - the Volcani Center, 7505101, Rishon LeZion, Israel.
N Argov-Argaman _ Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, 7610001, Rehovot, Israel.
We assessed the potential of phenolic compounds from Pistacia lentiscus (lentisk) to enhance production of milk constituents in bovine mammary epithelial cells (MEC). MEC were exposed to 0 (control), 1 or 10 ppm of polyphenols from lentisk ethanolic extract (PLEE) for 24 h. PLEE were absorbed by the MEC plasma membrane, but also penetrated the cell to accumulate in and around the nucleus. PLEE increased triglyceride content in the cell and its secretion to the medium, and significantly increased intracellular lipid droplet diameter. Compared to control, PLEE increased dose-dependently the lactose synthesis, secretion of whey proteins, and contents of casein. To evaluate mitochondrial activity under pro-oxidant load, MEC were preincubated with PLEE and exposed for 2 h to H2O2. Exposure to H2O2 increased the proportion of cells with impaired mitochondrial membrane potential twofold in controls, but not in PLEE-pre-treated cells. Accordingly, proton leakage was markedly decreased by PLEE, and coupling efficiency between the respiratory chain and ATP production was significantly enhanced. Thus, lentisk polyphenols divert energy to production of milk fat, protein and lactose, with less energy directed to cellular damage control; alternatively, PLEE enables MEC to maintain energy and oxidative status under extreme metabolic rate required for milk production and secretion, and reduces the limitation on energy required to support production.
