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פותח על ידי קלירמאש פתרונות בע"מ -
Vacuolar processing enzyme translocates to the vacuole through the autophagy pathway to induce programmed cell death
Year:
2020
Source of publication :
Autophagy
Authors :
אבו-עביד, מוחמד
;
.
אשל, דני
;
.
בלאוסוב, אדוארד
;
.
דניאלי, רז
;
.
שדות, עינת
;
.
Volume :
Co-Authors:

Paula Teper-Bamnolker   _Department of Postharvest and Food Sciences, The Volcani Center, ARO , Rishon LeZion, Israel.
Raz Danieli   _ Department of Postharvest and Food Sciences, The Volcani Center, ARO , Rishon LeZion, Israel; Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem , Rehovot 76100, Israel.
Hadas Peled-Zehavi  _ Department of Plant and Environmental Sciences, The Weizmann Institute of Science , Rehovot 76100, Israel. 
Eduard Belausov  _ Department of Ornamental Horticulture, The Volcani Center, ARO , Rishon LeZion, Israel. 
Mohamad Abu-Abied  _ Department of Ornamental Horticulture, The Volcani Center, ARO , Rishon LeZion, Israel.
Tamar Avin-Wittenberg   _ Department of Plant and Environmental Sciences, Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Givat Ram , Jerusalem, 9190401, Israel.
Einat Sadot   _ Department of Ornamental Horticulture, The Volcani Center, ARO , Rishon LeZion, Israel. 
Dani Eshel   _ Department of Postharvest and Food Sciences, The Volcani Center, ARO , Rishon LeZion, Israel.

Facilitators :
From page:
0
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Total pages:
1
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Abstract:

The caspase-like vacuolar processing enzyme (VPE) is a key factor in programmed cell death (PCD) associated with plant stress responses. Growth medium lacking a carbon source and dark conditions caused punctate labeling of 35S::VPE1-GFP (StVPE1-GFP) in potato leaves. Under conditions of carbon starvation, VPE activity and PCD symptoms strongly increased in BY-2 cells, but to a much lesser extent in VPE-RNAi BY-2 cells. During extended exposure to carbon starvation, VPE expression and activity levels peaked, with a gradual increase in BY-2 cell death. Histological analysis of StVPE1-GFP in BY-2 cells showed that carbon starvation induces its translocation from the endoplasmic reticulum to the central vacuole through tonoplast engulfment. Exposure of BY-2 culture to the macroautophagy/autophagy inhibitor concanamycin A led to, along with an accumulation of autophagic bodies, accumulation of StVPE1-GFP in the cell vacuole. This accumulation did not occur in the presence of 3-methyladenine, an inhibitor of early-stage autophagy. BY-2 cells constitutively expressing RFP-StATG8IL, an autophagosome marker, showed colocalization with the StVPE1-GFP protein in the cytoplasm and vacuole. RNAi silencing of the core autophagy component ATG4 in BY-2 cells reduced VPE activity and cell death. These results are the first to suggest that VPE translocates to the cell vacuole through the autophagy pathway, leading to PCD.

Note:
Related Files :
autophagosome
autophagy-related gene
BY-2 cells
carbon starvation
caspase-like protease
Solanum tuberosum
tobacco
עוד תגיות
תוכן קשור
More details
DOI :
10.1080/15548627.2020.1856492
Article number:
0
Affiliations:
Database:
PubMed
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
52086
Last updated date:
02/03/2022 17:27
Creation date:
07/12/2020 15:21
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Scientific Publication
Vacuolar processing enzyme translocates to the vacuole through the autophagy pathway to induce programmed cell death

Paula Teper-Bamnolker   _Department of Postharvest and Food Sciences, The Volcani Center, ARO , Rishon LeZion, Israel.
Raz Danieli   _ Department of Postharvest and Food Sciences, The Volcani Center, ARO , Rishon LeZion, Israel; Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem , Rehovot 76100, Israel.
Hadas Peled-Zehavi  _ Department of Plant and Environmental Sciences, The Weizmann Institute of Science , Rehovot 76100, Israel. 
Eduard Belausov  _ Department of Ornamental Horticulture, The Volcani Center, ARO , Rishon LeZion, Israel. 
Mohamad Abu-Abied  _ Department of Ornamental Horticulture, The Volcani Center, ARO , Rishon LeZion, Israel.
Tamar Avin-Wittenberg   _ Department of Plant and Environmental Sciences, Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Givat Ram , Jerusalem, 9190401, Israel.
Einat Sadot   _ Department of Ornamental Horticulture, The Volcani Center, ARO , Rishon LeZion, Israel. 
Dani Eshel   _ Department of Postharvest and Food Sciences, The Volcani Center, ARO , Rishon LeZion, Israel.

Vacuolar processing enzyme translocates to the vacuole through the autophagy pathway to induce programmed cell death

The caspase-like vacuolar processing enzyme (VPE) is a key factor in programmed cell death (PCD) associated with plant stress responses. Growth medium lacking a carbon source and dark conditions caused punctate labeling of 35S::VPE1-GFP (StVPE1-GFP) in potato leaves. Under conditions of carbon starvation, VPE activity and PCD symptoms strongly increased in BY-2 cells, but to a much lesser extent in VPE-RNAi BY-2 cells. During extended exposure to carbon starvation, VPE expression and activity levels peaked, with a gradual increase in BY-2 cell death. Histological analysis of StVPE1-GFP in BY-2 cells showed that carbon starvation induces its translocation from the endoplasmic reticulum to the central vacuole through tonoplast engulfment. Exposure of BY-2 culture to the macroautophagy/autophagy inhibitor concanamycin A led to, along with an accumulation of autophagic bodies, accumulation of StVPE1-GFP in the cell vacuole. This accumulation did not occur in the presence of 3-methyladenine, an inhibitor of early-stage autophagy. BY-2 cells constitutively expressing RFP-StATG8IL, an autophagosome marker, showed colocalization with the StVPE1-GFP protein in the cytoplasm and vacuole. RNAi silencing of the core autophagy component ATG4 in BY-2 cells reduced VPE activity and cell death. These results are the first to suggest that VPE translocates to the cell vacuole through the autophagy pathway, leading to PCD.

Scientific Publication
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