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פותח על ידי קלירמאש פתרונות בע"מ -
Sulfide-quinone reductase (SQR) of Rhodobacter capsulatus: expression, induction and inactivation
Year:
1998
Authors :
שחק, יוספה
;
.
Volume :
III
Co-Authors:

Schütz, M., Grieshabeck, C., Bronstein, M., Meldner, I., Padan, E. and Hauska, G.

Facilitators :
From page:
1919
To page:
1922
(
Total pages:
4
)
Abstract:

Inorganic reduced sulfur compounds serve as electron donors in many phototrophic and chemotrophic bacteria12. Sulfide-quinone reductase (SQR; E.C.1.8.5. “.) is one of the main enzymes involved in the oxidation of sulfide to sulfur. This sulfide induced membrane bound flavoprotein with an apparent MW of approximately 56-kDa was shown to be widely distributed among bacteria3. SQR was first purified from thylakoids of the cyanobacterium Oscillatoria limnetica4 and, more recently, from membranes of the ”non-sulfur“ purple bacterium Rb. capsulatus5. The sqr-gene of Rb. capsulatus had been sequenced and functionally expressed in E. coli5Rb. capsulatus oxidizes sulfide only to the redox level of sulfur. However, it is not known, whether SQR is the only sulfide oxidizing enzymatic system in this bacterium. Here we demonstrate that Rb. capsulatus looses its capability of sulfide dependent growth after inactivation of SQR. In former investigations it was shown that expression of SQR in Rb. capsulatus depends on sulfide and is possibly modulated by oxygen6. This results were achieved using a mutant strain with the genes of the luciferase of Vibrio fischeri (luxAB) inserted behind the putative sqrpromotor. However, the activity of the luciferase itself depends on oxygen. For this reason a second reporter gene, the alkaline phosphatase of E. coli (PhoA), was used in this investigation.

Note:
Related Files :
electron transport
genetic manipulation
photosynthesis
Purple Bacterium
Rhodobacter capsulatus
S metabolism
sulfide-quinone reductase (SQR)
עוד תגיות
תוכן קשור
More details
DOI :
https://doi.org/10.1007/978-94-011-3953-3_447
Article number:
0
Affiliations:
Database:
Publication Type:
פרק מתוך ספר
;
.
Language:
אנגלית
Editors' remarks:
ID:
52455
Last updated date:
02/03/2022 17:27
Creation date:
20/12/2020 10:30
Scientific Publication
Sulfide-quinone reductase (SQR) of Rhodobacter capsulatus: expression, induction and inactivation
III

Schütz, M., Grieshabeck, C., Bronstein, M., Meldner, I., Padan, E. and Hauska, G.

Sulfide-quinone reductase (SQR) of Rhodobacter capsulatus: expression, induction and inactivation .

Inorganic reduced sulfur compounds serve as electron donors in many phototrophic and chemotrophic bacteria12. Sulfide-quinone reductase (SQR; E.C.1.8.5. “.) is one of the main enzymes involved in the oxidation of sulfide to sulfur. This sulfide induced membrane bound flavoprotein with an apparent MW of approximately 56-kDa was shown to be widely distributed among bacteria3. SQR was first purified from thylakoids of the cyanobacterium Oscillatoria limnetica4 and, more recently, from membranes of the ”non-sulfur“ purple bacterium Rb. capsulatus5. The sqr-gene of Rb. capsulatus had been sequenced and functionally expressed in E. coli5Rb. capsulatus oxidizes sulfide only to the redox level of sulfur. However, it is not known, whether SQR is the only sulfide oxidizing enzymatic system in this bacterium. Here we demonstrate that Rb. capsulatus looses its capability of sulfide dependent growth after inactivation of SQR. In former investigations it was shown that expression of SQR in Rb. capsulatus depends on sulfide and is possibly modulated by oxygen6. This results were achieved using a mutant strain with the genes of the luciferase of Vibrio fischeri (luxAB) inserted behind the putative sqrpromotor. However, the activity of the luciferase itself depends on oxygen. For this reason a second reporter gene, the alkaline phosphatase of E. coli (PhoA), was used in this investigation.

Scientific Publication
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