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פותח על ידי קלירמאש פתרונות בע"מ -
Temporal Resistome and Microbial Community Dynamics in an Intensive Aquaculture Facility with Prophylactic Antimicrobial Treatment
Year:
2020
Authors :
גטיקה, ג'ואאו
;
.
דרור, ברק
;
.
זולטי, אביחי
;
.
מינץ, דרור
;
.
סיטרין, אדי
;
.
פלטי, יוסף
;
.
Volume :
Co-Authors:

Hemant J Patil _ Institute of Soil, Water and Environmental Sciences, Volcani Center, Agricultural Research Organization, P.O. Box 15159, RishonLezion 7528809, Israel.
Joao Gatica  _ Institute of Soil, Water and Environmental Sciences, Volcani Center, Agricultural Research Organization, P.O. Box 15159, RishonLezion 7528809, Israel; Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 91905, Israel.
Avihai Zolti  _ Institute of Soil, Water and Environmental Sciences, Volcani Center, Agricultural Research Organization, P.O. Box 15159, RishonLezion 7528809, Israel; Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 91905, Israel.
Ayana Benet-Perelberg  _ Dor Aquaculture Research Station, Fisheries Department, Israel Ministry of Agriculture and Rural Development, Dor 3082000, Israel.
Alon Naor  _ Dor Aquaculture Research Station, Fisheries Department, Israel Ministry of Agriculture and Rural Development, Dor 3082000, Israel.
Barak Dror  _ Institute of Soil, Water and Environmental Sciences, Volcani Center, Agricultural Research Organization, P.O. Box 15159, RishonLezion 7528809, Israel.1 2
Ashraf Al Ashhab  _ The Dead Sea and Arava Science Center, Masada 86900, Israel; Department of Marine Biology, Leon H. Charney School of Marine Sciences, University of Haifa, Haifa 3498838, Israel.
Sophi Marman  _ Department of Marine Biology, Leon H. Charney School of Marine Sciences, University of Haifa, Haifa 3498838, Israel.
Nur A Hasan  _  CosmosID Inc., Rockville, MD 20742, USA; Center for Bioinformatics and Computational Biology, University of Maryland, College Park, MD 20742, USA.
Rita R Colwell  _  CosmosID Inc., Rockville, MD 20742, USA; Center for Bioinformatics and Computational Biology, University of Maryland, College Park, MD 20742, USA; Maryland Pathogen Research Institute, University of Maryland, College Park, MD 20742, USA..
Daniel Sher  _ Department of Marine Biology, Leon H. Charney School of Marine Sciences, University of Haifa, Haifa 3498838, Israel.
Dror Minz  _ Institute of Soil, Water and Environmental Sciences, Volcani Center, Agricultural Research Organization, P.O. Box 15159, RishonLezion 7528809, Israel.
Eddie Cytryn  _ Institute of Soil, Water and Environmental Sciences, Volcani Center, Agricultural Research Organization, P.O. Box 15159, RishonLezion 7528809, Israel.

 

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Total pages:
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Abstract:

Excessive use of antimicrobials in aquaculture is concerning, given possible environmental ramifications and the potential contribution to the spread of antimicrobial resistance (AR). In this study, we explored seasonal abundance of antimicrobial resistance genes and bacterial community composition in the water column of an intensive aquaculture pond stocked with Silver Carp (Hypophthalmichthys molitrix) prophylactically treated with sulfamethoprim (25% sulfadiazine; 5% trimethoprim), relative to an adjacent unstocked reservoir. Bacterial community composition was monitored using high-throughput sequencing of 16S rRNA gene amplicons in eight sampling profiles to determine seasonal dynamics, representing principal stages in the fish fattening cycle. In tandem, qPCR was applied to assess relative abundance of selected antimicrobial resistance genes (sul1, sul2, dfrA1, tetA and blaTEM) and class-1 integrons (int1). Concomitantly, resistomes were extrapolated from shotgun metagenomes in representative profiles. Analyses revealed increased relative abundance of sulfonamide and tetracycline resistance genes in fishpond-03, relative to pre-stocking and reservoir levels, whereas no significant differences were observed for genes encoding resistance to antimicrobials that were not used in the fishpond-03. Seasons strongly dictated bacterial community composition, with high abundance of cyanobacteria in summer and increased relative abundance of Flavobacterium in the winter. Our results indicate that prophylactic use of sulfonamides in intensive aquaculture ponds facilitates resistance suggesting that prophylactic use of these antimicrobials in aquaculture should be restricted.

Note:
Related Files :
Antimicrobial
antimicrobial resistance
aquaculture
Metagenome
microbiome
Resistome
עוד תגיות
תוכן קשור
More details
DOI :
10.3390/microorganisms8121984
Article number:
0
Affiliations:
Database:
PubMed
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
52498
Last updated date:
02/03/2022 17:27
Creation date:
20/12/2020 17:57
Scientific Publication
Temporal Resistome and Microbial Community Dynamics in an Intensive Aquaculture Facility with Prophylactic Antimicrobial Treatment

Hemant J Patil _ Institute of Soil, Water and Environmental Sciences, Volcani Center, Agricultural Research Organization, P.O. Box 15159, RishonLezion 7528809, Israel.
Joao Gatica  _ Institute of Soil, Water and Environmental Sciences, Volcani Center, Agricultural Research Organization, P.O. Box 15159, RishonLezion 7528809, Israel; Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 91905, Israel.
Avihai Zolti  _ Institute of Soil, Water and Environmental Sciences, Volcani Center, Agricultural Research Organization, P.O. Box 15159, RishonLezion 7528809, Israel; Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 91905, Israel.
Ayana Benet-Perelberg  _ Dor Aquaculture Research Station, Fisheries Department, Israel Ministry of Agriculture and Rural Development, Dor 3082000, Israel.
Alon Naor  _ Dor Aquaculture Research Station, Fisheries Department, Israel Ministry of Agriculture and Rural Development, Dor 3082000, Israel.
Barak Dror  _ Institute of Soil, Water and Environmental Sciences, Volcani Center, Agricultural Research Organization, P.O. Box 15159, RishonLezion 7528809, Israel.1 2
Ashraf Al Ashhab  _ The Dead Sea and Arava Science Center, Masada 86900, Israel; Department of Marine Biology, Leon H. Charney School of Marine Sciences, University of Haifa, Haifa 3498838, Israel.
Sophi Marman  _ Department of Marine Biology, Leon H. Charney School of Marine Sciences, University of Haifa, Haifa 3498838, Israel.
Nur A Hasan  _  CosmosID Inc., Rockville, MD 20742, USA; Center for Bioinformatics and Computational Biology, University of Maryland, College Park, MD 20742, USA.
Rita R Colwell  _  CosmosID Inc., Rockville, MD 20742, USA; Center for Bioinformatics and Computational Biology, University of Maryland, College Park, MD 20742, USA; Maryland Pathogen Research Institute, University of Maryland, College Park, MD 20742, USA..
Daniel Sher  _ Department of Marine Biology, Leon H. Charney School of Marine Sciences, University of Haifa, Haifa 3498838, Israel.
Dror Minz  _ Institute of Soil, Water and Environmental Sciences, Volcani Center, Agricultural Research Organization, P.O. Box 15159, RishonLezion 7528809, Israel.
Eddie Cytryn  _ Institute of Soil, Water and Environmental Sciences, Volcani Center, Agricultural Research Organization, P.O. Box 15159, RishonLezion 7528809, Israel.

 

Temporal Resistome and Microbial Community Dynamics in an Intensive Aquaculture Facility with Prophylactic Antimicrobial Treatment

Excessive use of antimicrobials in aquaculture is concerning, given possible environmental ramifications and the potential contribution to the spread of antimicrobial resistance (AR). In this study, we explored seasonal abundance of antimicrobial resistance genes and bacterial community composition in the water column of an intensive aquaculture pond stocked with Silver Carp (Hypophthalmichthys molitrix) prophylactically treated with sulfamethoprim (25% sulfadiazine; 5% trimethoprim), relative to an adjacent unstocked reservoir. Bacterial community composition was monitored using high-throughput sequencing of 16S rRNA gene amplicons in eight sampling profiles to determine seasonal dynamics, representing principal stages in the fish fattening cycle. In tandem, qPCR was applied to assess relative abundance of selected antimicrobial resistance genes (sul1, sul2, dfrA1, tetA and blaTEM) and class-1 integrons (int1). Concomitantly, resistomes were extrapolated from shotgun metagenomes in representative profiles. Analyses revealed increased relative abundance of sulfonamide and tetracycline resistance genes in fishpond-03, relative to pre-stocking and reservoir levels, whereas no significant differences were observed for genes encoding resistance to antimicrobials that were not used in the fishpond-03. Seasons strongly dictated bacterial community composition, with high abundance of cyanobacteria in summer and increased relative abundance of Flavobacterium in the winter. Our results indicate that prophylactic use of sulfonamides in intensive aquaculture ponds facilitates resistance suggesting that prophylactic use of these antimicrobials in aquaculture should be restricted.

Scientific Publication
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