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אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
IFN-γ Regulation of Vacuolar pH, Cathepsin D Processing and Autophagy in Mammary Epithelial Cells
Year:
2008
Source of publication :
Journal of Cellular Biochemistry
Authors :
ראובני, משה
;
.
Volume :
105 (1)
Co-Authors:

Khalkhali-Ellis, Z.; Abbott, D.; Bailey, C.; Goossens, W.; Margaryan, N.; Gluck, S. and Hendrix, M.

Facilitators :
From page:
208
To page:
218
(
Total pages:
11
)
Abstract:

In this study we examined the ability of interferon‐γ (IFN‐γ) to regulate mammary epithelial cell growth and gene expression, with particular emphasis on two genes: Maspin (a member of serine protease inhibitor superfamily), and the lysosomal aspartyl endopeptidase cathepsin D (CatD). The protein products of these genes are critically involved in regulation of multitude of biological functions in different stages of mammary tissue development and remodeling. In addition, the expression of Maspin is down‐regulated in primary breast cancer and is lost in metastatic disease, while CatD is excessively produced and aberrantly secreted by breast cancer cells. We report that IFN‐γ receptors are expressed in mammary epithelial cells, and receptor engagement by IFN‐γ transduces the IFN‐γ signal via Stat‐1 resulting in decreased vacuolar pH. This change in vacuolar pH alters CatD protein processing and secretion concurrent with increased Maspin secretion. In addition, IFN‐γ exerts a suppressive effect on cell growth and proliferation, and induces morphological changes in mammary epithelial cells. Our studies also reveal that breast cancer cells, which are devoid of Maspin, are refractory to IFN‐γ with respect to changes in vacuolar pH and CatD. However, Maspin transfection of breast cancer cells partially sensitizes the cells to IFN‐γ's effect, thus providing new therapeutic implications. J. Cell. Biochem. 105: 208–218, 2008. © 2008 Wiley‐Liss, Inc.

Note:
Related Files :
autophagy
breast cancer
cancer (disease)
Cathepsin D
IFN‐γ
Mammary epithelial cell
Maspin
vATPase
עוד תגיות
תוכן קשור
More details
DOI :
https://doi.org/10.1002/jcb.21814
Article number:
0
Affiliations:
Database:
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
52725
Last updated date:
02/03/2022 17:27
Creation date:
28/12/2020 14:44
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Scientific Publication
IFN-γ Regulation of Vacuolar pH, Cathepsin D Processing and Autophagy in Mammary Epithelial Cells
105 (1)

Khalkhali-Ellis, Z.; Abbott, D.; Bailey, C.; Goossens, W.; Margaryan, N.; Gluck, S. and Hendrix, M.

IFN-γ Regulation of Vacuolar pH, Cathepsin D Processing and Autophagy in Mammary Epithelial Cells

In this study we examined the ability of interferon‐γ (IFN‐γ) to regulate mammary epithelial cell growth and gene expression, with particular emphasis on two genes: Maspin (a member of serine protease inhibitor superfamily), and the lysosomal aspartyl endopeptidase cathepsin D (CatD). The protein products of these genes are critically involved in regulation of multitude of biological functions in different stages of mammary tissue development and remodeling. In addition, the expression of Maspin is down‐regulated in primary breast cancer and is lost in metastatic disease, while CatD is excessively produced and aberrantly secreted by breast cancer cells. We report that IFN‐γ receptors are expressed in mammary epithelial cells, and receptor engagement by IFN‐γ transduces the IFN‐γ signal via Stat‐1 resulting in decreased vacuolar pH. This change in vacuolar pH alters CatD protein processing and secretion concurrent with increased Maspin secretion. In addition, IFN‐γ exerts a suppressive effect on cell growth and proliferation, and induces morphological changes in mammary epithelial cells. Our studies also reveal that breast cancer cells, which are devoid of Maspin, are refractory to IFN‐γ with respect to changes in vacuolar pH and CatD. However, Maspin transfection of breast cancer cells partially sensitizes the cells to IFN‐γ's effect, thus providing new therapeutic implications. J. Cell. Biochem. 105: 208–218, 2008. © 2008 Wiley‐Liss, Inc.

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