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פותח על ידי קלירמאש פתרונות בע"מ -
Mitochondrial DNA Polymerase from germinating Bean Rust Uredospores
Year:
1978
Source of publication :
Experimental Mycology
Authors :
יניב, זהרה
;
.
Volume :
2
Co-Authors:

R.C. Staples

Facilitators :
From page:
290
To page:
294
(
Total pages:
5
)
Abstract:

Several DNA-dependent DNA polymerases were extracted and purified from uredospores of the bean rust fungus after germination for 4 h on water. Under such conditions, only simple germ tubes developed, and nuclear division did not occur. The mitochondrial enzyme from germinated spores had a molecular weight of 150,000 but was resolved into two fractions by isoelectric focusing: a more abundant fraction at pH 5.4 and a minor protein at pH 6.3. These enzymes were inhibited by N-ethylmaleimide (NEM).

Note:
Related Files :
Differentiation
DNA
dna polymerase
fungi
infection structures
isoelectric focusing
mitochondria
Rust fungus
Spore germination
עוד תגיות
תוכן קשור
More details
DOI :
https://doi.org/10.1016/S0147-5975(78)80022-X
Article number:
0
Affiliations:
Database:
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
53020
Last updated date:
02/03/2022 17:27
Creation date:
10/01/2021 10:49
Scientific Publication
Mitochondrial DNA Polymerase from germinating Bean Rust Uredospores
2

R.C. Staples

Mitochondrial DNA Polymerase from germinating Bean Rust Uredospores

Several DNA-dependent DNA polymerases were extracted and purified from uredospores of the bean rust fungus after germination for 4 h on water. Under such conditions, only simple germ tubes developed, and nuclear division did not occur. The mitochondrial enzyme from germinated spores had a molecular weight of 150,000 but was resolved into two fractions by isoelectric focusing: a more abundant fraction at pH 5.4 and a minor protein at pH 6.3. These enzymes were inhibited by N-ethylmaleimide (NEM).

Scientific Publication
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