Seeds from four Cruciferae species, native to Israel, have been examined for fatty acid composition. The goal was to identify from the wild gene collection species rich in fatty acids useful for industry. Species rich in emcic acid (C22:1), such as species of Crambe and Sinapis, are low in polyunsaturated linolenic acid (C18:3) as well as in the saturated palmitic acid (C16:O). The same negative correlation is observed in species low in erucic acid (species of Sisymbrium and Lepidium), in which 44% and 42% linolenic acid was found, respectively. No relation was found between the content of erucic and oleic acids, as opposed to the situation in cultivated rape seeds.