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פותח על ידי קלירמאש פתרונות בע"מ -
A De Novo Transcriptomics Approach Reveals Genes Involved in Thrips Tabaci Resistance to Spinosad
Year:
2021
Source of publication :
Insects (journal)
Authors :
בן-יקיר, דוד
;
.
גנאים, מוראד
;
.
לבדב, גלינה
;
.
קונצדלוב, סבטלנה
;
.
רוזן, רן
;
.
Volume :
Co-Authors:

Ran Rosen  _  Department of Entomology, Volcani Center, Rishon LeZion 7505101, Israel. 
Galina Lebedev   _ Department of Entomology, Volcani Center, Rishon LeZion 7505101, Israel.
Svetlana Kontsedalov   _ Department of Entomology, Volcani Center, Rishon LeZion 7505101, Israel. 
David Ben-Yakir   _ Department of Entomology, Volcani Center, Rishon LeZion 7505101, Israel.
Murad Ghanim  _ Department of Entomology, Volcani Center, Rishon LeZion 7505101, Israel.

Facilitators :
From page:
0
To page:
0
(
Total pages:
1
)
Abstract:

The onion thrip, Thrips tabaci (Thysanoptera: Thripidae) is a major polyphagous pest that attacks a wide range of economically important crops, especially Allium species. The thrip's damage can result in yield loss of up to 60% in onions (Allium cepa). In the past few decades, thrip resistance to insecticides with various modes of actions have been documented. These include resistance to spinosad, a major active compound used against thrips, which was reported from Israel. Little is known about the molecular mechanisms underlying spinosad resistance in T. tabaci. We attempted to characterize the mechanisms involved in resistance to spinosad using quantitative transcriptomics. Susceptible (LC50 = 0.6 ppm) and resistant (LC50 = 23,258 ppm) thrip populations were collected from Israel. An additional resistant population (LC50 = 117 ppm) was selected in the laboratory from the susceptible population. De novo transcriptome analysis on the resistant and susceptible population was conducted to identify differently expressed genes (DGEs) that might be involved in the resistance against spinosad. In this analysis, 25,552 unigenes were sequenced, assembled, and functionally annotated, and more than 1500 DGEs were identified. The expression levels of candidate genes, which included cytochrome P450 and vittelogenin, were validated using quantitative RT-PCR. The cytochrome P450 expression gradually increased with the increase of the resistance. Higher expression levels of vitellogenin in the resistant populations were correlated with higher fecundity, suggesting a positive effect of the resistance on resistant populations. This research provides a novel genetic resource for onion thrips and a comprehensive molecular examination of resistant populations to spinosad. Those resources are important for future studies concerning thrips and resistance in insect pests regarding agriculture.

Note:
Related Files :
Fitness cost
Resistance
Spinosad
Thrips tabaci
transcriptome
עוד תגיות
תוכן קשור
More details
DOI :
10.3390/insects12010067
Article number:
0
Affiliations:
Database:
PubMed
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
53150
Last updated date:
02/03/2022 17:27
Creation date:
20/01/2021 17:07
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Scientific Publication
A De Novo Transcriptomics Approach Reveals Genes Involved in Thrips Tabaci Resistance to Spinosad

Ran Rosen  _  Department of Entomology, Volcani Center, Rishon LeZion 7505101, Israel. 
Galina Lebedev   _ Department of Entomology, Volcani Center, Rishon LeZion 7505101, Israel.
Svetlana Kontsedalov   _ Department of Entomology, Volcani Center, Rishon LeZion 7505101, Israel. 
David Ben-Yakir   _ Department of Entomology, Volcani Center, Rishon LeZion 7505101, Israel.
Murad Ghanim  _ Department of Entomology, Volcani Center, Rishon LeZion 7505101, Israel.

A De Novo Transcriptomics Approach Reveals Genes Involved in Thrips Tabaci Resistance to Spinosad .

The onion thrip, Thrips tabaci (Thysanoptera: Thripidae) is a major polyphagous pest that attacks a wide range of economically important crops, especially Allium species. The thrip's damage can result in yield loss of up to 60% in onions (Allium cepa). In the past few decades, thrip resistance to insecticides with various modes of actions have been documented. These include resistance to spinosad, a major active compound used against thrips, which was reported from Israel. Little is known about the molecular mechanisms underlying spinosad resistance in T. tabaci. We attempted to characterize the mechanisms involved in resistance to spinosad using quantitative transcriptomics. Susceptible (LC50 = 0.6 ppm) and resistant (LC50 = 23,258 ppm) thrip populations were collected from Israel. An additional resistant population (LC50 = 117 ppm) was selected in the laboratory from the susceptible population. De novo transcriptome analysis on the resistant and susceptible population was conducted to identify differently expressed genes (DGEs) that might be involved in the resistance against spinosad. In this analysis, 25,552 unigenes were sequenced, assembled, and functionally annotated, and more than 1500 DGEs were identified. The expression levels of candidate genes, which included cytochrome P450 and vittelogenin, were validated using quantitative RT-PCR. The cytochrome P450 expression gradually increased with the increase of the resistance. Higher expression levels of vitellogenin in the resistant populations were correlated with higher fecundity, suggesting a positive effect of the resistance on resistant populations. This research provides a novel genetic resource for onion thrips and a comprehensive molecular examination of resistant populations to spinosad. Those resources are important for future studies concerning thrips and resistance in insect pests regarding agriculture.

Scientific Publication
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