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פותח על ידי קלירמאש פתרונות בע"מ -
Enhanced Production of Aromatic Amino Acids in Tobacco Plants Leads to Increased Phenylpropanoid Metabolites and Tolerance to Stresses
Year:
2021
Source of publication :
Frontiers in Plant Science
Authors :
דור, יבגניה
;
.
Volume :
Co-Authors:

Moran Oliva  _ Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel.
Aviv Guy  _  Laboratory of Plant Science, MIGAL - Galilee Research Institute, Kiryat Shmona, Israel.
Gad Galili  _ Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel.
Evgenia Dor  _ Department of Weed Research, Agriculture Research Organization, Newe Ya'ar Research Center, The Volcani Center, Ramat Yishay, Israel.
Ron Schweitzer _ Tel-Hai College, Upper Galilee, Israel.
Rachel Amir  _ Laboratory of Plant Science, MIGAL - Galilee Research Institute, Kiryat Shmona, Israel; Tel-Hai College, Upper Galilee, Israel.
Yael Hacham  _ Laboratory of Plant Science, MIGAL - Galilee Research Institute, Kiryat Shmona, Israel; Tel-Hai College, Upper Galilee, Israel.

 

Facilitators :
From page:
0
To page:
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Total pages:
1
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Abstract:

Aromatic amino acids (AAAs) synthesized in plants via the shikimate pathway can serve as precursors for a wide range of secondary metabolites that are important for plant defense. The goals of the current study were to test the effect of increased AAAs on primary and secondary metabolic profiles and to reveal whether these plants are more tolerant to abiotic stresses (oxidative, drought and salt) and to Phelipanche egyptiaca (Egyptian broomrape), an obligate parasitic plant. To this end, tobacco (Nicotiana tabacum) plants were transformed with a bacterial gene (AroG) encode to feedback-insensitive 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase, the first enzyme of the shikimate pathway. Two sets of transgenic plants were obtained: the first had low expression of the AroG protein, a normal phenotype and minor metabolic changes; the second had high accumulation of the AroG protein with normal, or deleterious morphological changes having a dramatic shift in plant metabolism. Metabolic profiling analysis revealed that the leaves of the transgenic plants had increased levels of phenylalanine (up to 43-fold), tyrosine (up to 24-fold) and tryptophan (up to 10-fold) compared to control plants having an empty vector (EV) and wild type (WT) plants. The significant increase in phenylalanine was accompanied by higher levels of metabolites that belong to the phenylpropanoid pathway. AroG plants showed improved tolerance to salt stress but not to oxidative or drought stress. The most significant improved tolerance was to P. aegyptiaca. Unlike WT/EV plants that were heavily infected by the parasite, the transgenic AroG plants strongly inhibited P. aegyptiaca development, and only a few stems of the parasite appeared above the soil. This delayed development of P. aegyptiaca could be the result of higher accumulation of several phenylpropanoids in the transgenic AroG plants and in P. aegyptiaca, that apparently affected its growth. These findings indicate that high levels of AAAs and their related metabolites have the potential of controlling the development of parasitic plants.

Note:
Related Files :
aroG
aromatic amino-acids
parasitic plant
Phelipanche aegyptiaca
salt stress
tobacco (Nicotiana tabacum)
עוד תגיות
תוכן קשור
More details
DOI :
10.3389/fpls.2020.604349
Article number:
0
Affiliations:
Database:
PubMed
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
53331
Last updated date:
02/03/2022 17:27
Creation date:
01/02/2021 18:22
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Scientific Publication
Enhanced Production of Aromatic Amino Acids in Tobacco Plants Leads to Increased Phenylpropanoid Metabolites and Tolerance to Stresses

Moran Oliva  _ Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel.
Aviv Guy  _  Laboratory of Plant Science, MIGAL - Galilee Research Institute, Kiryat Shmona, Israel.
Gad Galili  _ Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel.
Evgenia Dor  _ Department of Weed Research, Agriculture Research Organization, Newe Ya'ar Research Center, The Volcani Center, Ramat Yishay, Israel.
Ron Schweitzer _ Tel-Hai College, Upper Galilee, Israel.
Rachel Amir  _ Laboratory of Plant Science, MIGAL - Galilee Research Institute, Kiryat Shmona, Israel; Tel-Hai College, Upper Galilee, Israel.
Yael Hacham  _ Laboratory of Plant Science, MIGAL - Galilee Research Institute, Kiryat Shmona, Israel; Tel-Hai College, Upper Galilee, Israel.

 

Enhanced Production of Aromatic Amino Acids in Tobacco Plants Leads to Increased Phenylpropanoid Metabolites and Tolerance to Stresses

Aromatic amino acids (AAAs) synthesized in plants via the shikimate pathway can serve as precursors for a wide range of secondary metabolites that are important for plant defense. The goals of the current study were to test the effect of increased AAAs on primary and secondary metabolic profiles and to reveal whether these plants are more tolerant to abiotic stresses (oxidative, drought and salt) and to Phelipanche egyptiaca (Egyptian broomrape), an obligate parasitic plant. To this end, tobacco (Nicotiana tabacum) plants were transformed with a bacterial gene (AroG) encode to feedback-insensitive 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase, the first enzyme of the shikimate pathway. Two sets of transgenic plants were obtained: the first had low expression of the AroG protein, a normal phenotype and minor metabolic changes; the second had high accumulation of the AroG protein with normal, or deleterious morphological changes having a dramatic shift in plant metabolism. Metabolic profiling analysis revealed that the leaves of the transgenic plants had increased levels of phenylalanine (up to 43-fold), tyrosine (up to 24-fold) and tryptophan (up to 10-fold) compared to control plants having an empty vector (EV) and wild type (WT) plants. The significant increase in phenylalanine was accompanied by higher levels of metabolites that belong to the phenylpropanoid pathway. AroG plants showed improved tolerance to salt stress but not to oxidative or drought stress. The most significant improved tolerance was to P. aegyptiaca. Unlike WT/EV plants that were heavily infected by the parasite, the transgenic AroG plants strongly inhibited P. aegyptiaca development, and only a few stems of the parasite appeared above the soil. This delayed development of P. aegyptiaca could be the result of higher accumulation of several phenylpropanoids in the transgenic AroG plants and in P. aegyptiaca, that apparently affected its growth. These findings indicate that high levels of AAAs and their related metabolites have the potential of controlling the development of parasitic plants.

Scientific Publication
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