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פותח על ידי קלירמאש פתרונות בע"מ -
A transgenic RNAi approach for developing tomato plants immune to Pepino mosaic virus
Year:
2021
Source of publication :
Plant Pathology
Authors :
גל-און, עמית
;
.
וולף, דליה
;
.
ליבמן, דיאנה
;
.
Volume :
Co-Authors:
  • Leibman, D.
  • Ortega-Parra, N.
  • Wolf, D.
  • Shterkman, M.
  • Hanssen, I.
  • Gal-On, A.
Facilitators :
From page:
1
To page:
10
(
Total pages:
10
)
Abstract:

RNA interference (RNAi) or gene silencing is a natural defence response of plants to invading viruses. Here, we applied this approach against pepino mosaic virus (PepMV) isolates in their natural host, tomato. PepMV isolates differ in their genetic sequences, the severity of the disease they induce, and their worldwide distribution. PepMV causes heavy crop losses, mainly due to impaired tomato fruit quality. Resistant varieties are not yet available, despite many years of resistance breeding efforts within the tomato seed industry. To generate broad resistance to PepMV strains, conserved sequences from three different strains of PepMV (US1, LP, and CH2) were synthesized as a single insert and cloned in a hairpin configuration into a binary vector, which was used to transform tomato plants. Transgenic tomato lines that expressed a high level of transgene-siRNA exhibited immunity to PepMV strains, including a new Israeli isolate. This immunity was maintained even after graft inoculation, in which a transgenic scion was grafted onto nontransgenic infected rootstocks. However, an immune transgenic rootstock was unable to induce resistance in a nontransformed scion. These results provide the first example of engineered immunity to diverse PepMV strains in transgenic tomato based on gene silencing.

Note:
Related Files :
immunity
Pepino mosaic virus
tomato
Transgenic
עוד תגיות
תוכן קשור
More details
DOI :
10.1111/ppa.13346
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
53544
Last updated date:
02/03/2022 17:27
Creation date:
18/02/2021 01:16
Scientific Publication
A transgenic RNAi approach for developing tomato plants immune to Pepino mosaic virus
  • Leibman, D.
  • Ortega-Parra, N.
  • Wolf, D.
  • Shterkman, M.
  • Hanssen, I.
  • Gal-On, A.
A transgenic RNAi approach for developing tomato plants immune to Pepino mosaic virus

RNA interference (RNAi) or gene silencing is a natural defence response of plants to invading viruses. Here, we applied this approach against pepino mosaic virus (PepMV) isolates in their natural host, tomato. PepMV isolates differ in their genetic sequences, the severity of the disease they induce, and their worldwide distribution. PepMV causes heavy crop losses, mainly due to impaired tomato fruit quality. Resistant varieties are not yet available, despite many years of resistance breeding efforts within the tomato seed industry. To generate broad resistance to PepMV strains, conserved sequences from three different strains of PepMV (US1, LP, and CH2) were synthesized as a single insert and cloned in a hairpin configuration into a binary vector, which was used to transform tomato plants. Transgenic tomato lines that expressed a high level of transgene-siRNA exhibited immunity to PepMV strains, including a new Israeli isolate. This immunity was maintained even after graft inoculation, in which a transgenic scion was grafted onto nontransgenic infected rootstocks. However, an immune transgenic rootstock was unable to induce resistance in a nontransformed scion. These results provide the first example of engineered immunity to diverse PepMV strains in transgenic tomato based on gene silencing.

Scientific Publication
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