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Fine Mapping of the "black" Peel Color in Pomegranate ( Punica granatum L.) Strongly Suggests That a Mutation in the Anthocyanidin Reductase ( ANR) Gene Is Responsible for the Trait Fine Mapping of the "black" Peel Color in Pomegranate ( Punica granatum L.) Strongly Suggests That a Mutation in the Anthocyanidin Reductase ( ANR) Gene Is Responsible for the Trait
Year:
2021
Source of publication :
Frontiers in Plant Science
Authors :
אופיר, רון
;
.
בר-יעקב, עירית
;
.
דורון-פייגנבאום, עדי
;
.
הולנד, דורון
;
.
הראל-ביז'ה, רותם
;
.
טריינין, טלי
;
.
שרמן, עמיר
;
.
Volume :
Co-Authors:

Taly Trainin 
 Rotem Harel-Beja 
 Irit Bar-Ya'akov 
 Zohar Ben-Simhon 
 Rami Yahalomi 
 Hamutal Borochov-Neori 
 Ron Ophir 
 Amir Sherman 
 Adi Doron-Faigenboim 
 Doron Holland              

Facilitators :
From page:
0
To page:
0
(
Total pages:
1
)
Abstract:

Anthocyanins are important dietary and health-promoting substances present in high quantities in the peel and arils of the pomegranate (Punica granatum L.) fruit. Yet, there is a high variation in the content of anthocyanin among different pomegranate varieties. The 'Black' pomegranate variety (P.G.127-28) found in Israel contains exceptionally high levels of anthocyanins in its fruit peel which can reach up to two orders of magnitude higher content as compared to that of other pomegranate varieties' peel anthocyanins. Biochemical analysis reveals that delphinidin is highly abundant in the peel of 'Black' variety. The pattern of anthocyanin accumulation in the fruit peel during fruit development of 'Black' variety differs from that of other pomegranates. High anthocyanin levels are maintained during all developmental stages. Moreover, the accumulation of anthocyanin in the fruit peel of 'Black' variety is not dependent on light. Genetic analysis of an F2 population segregating for the "black" phenotype reveals that it is determined by a single recessive gene. Genetic mapping of the F2 population using single nucleotide polymorphism (SNP) markers identified few markers tightly linked to the "black" phenotype. Recombination analysis of the F2 population and F3 populations narrowed the "black" trait to an area of 178.5 kb on the draft genome sequence of pomegranate cv. 'Dabenzi.' A putative anthocyanidin reductase (ANR) gene is located in this area. Only pomegranate varieties displaying the "black" trait carry a base pair deletion toward the end of the gene, causing a frame shift resulting in a shorter protein. We propose that this mutation in the ANR gene is responsible for the different anthocyanin composition and high anthocyanin levels of the "black" trait in pomegranate.

 

Note:
Related Files :
anthocyanidin reductase
Anthocyanins
fruit
Genetic mapping
pomegranate genetic variability
Punica granatum
עוד תגיות
תוכן קשור
More details
DOI :
10.3389/fpls.2021.642019
Article number:
0
Affiliations:
Database:
PubMed
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
54318
Last updated date:
02/03/2022 17:27
Creation date:
21/03/2021 18:21
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Scientific Publication
Fine Mapping of the "black" Peel Color in Pomegranate ( Punica granatum L.) Strongly Suggests That a Mutation in the Anthocyanidin Reductase ( ANR) Gene Is Responsible for the Trait Fine Mapping of the "black" Peel Color in Pomegranate ( Punica granatum L.) Strongly Suggests That a Mutation in the Anthocyanidin Reductase ( ANR) Gene Is Responsible for the Trait

Taly Trainin 
 Rotem Harel-Beja 
 Irit Bar-Ya'akov 
 Zohar Ben-Simhon 
 Rami Yahalomi 
 Hamutal Borochov-Neori 
 Ron Ophir 
 Amir Sherman 
 Adi Doron-Faigenboim 
 Doron Holland              

Fine Mapping of the "black" Peel Color in Pomegranate ( Punica granatum L.) Strongly Suggests That a Mutation in the Anthocyanidin Reductase ( ANR) Gene Is Responsible for the Trait .

Anthocyanins are important dietary and health-promoting substances present in high quantities in the peel and arils of the pomegranate (Punica granatum L.) fruit. Yet, there is a high variation in the content of anthocyanin among different pomegranate varieties. The 'Black' pomegranate variety (P.G.127-28) found in Israel contains exceptionally high levels of anthocyanins in its fruit peel which can reach up to two orders of magnitude higher content as compared to that of other pomegranate varieties' peel anthocyanins. Biochemical analysis reveals that delphinidin is highly abundant in the peel of 'Black' variety. The pattern of anthocyanin accumulation in the fruit peel during fruit development of 'Black' variety differs from that of other pomegranates. High anthocyanin levels are maintained during all developmental stages. Moreover, the accumulation of anthocyanin in the fruit peel of 'Black' variety is not dependent on light. Genetic analysis of an F2 population segregating for the "black" phenotype reveals that it is determined by a single recessive gene. Genetic mapping of the F2 population using single nucleotide polymorphism (SNP) markers identified few markers tightly linked to the "black" phenotype. Recombination analysis of the F2 population and F3 populations narrowed the "black" trait to an area of 178.5 kb on the draft genome sequence of pomegranate cv. 'Dabenzi.' A putative anthocyanidin reductase (ANR) gene is located in this area. Only pomegranate varieties displaying the "black" trait carry a base pair deletion toward the end of the gene, causing a frame shift resulting in a shorter protein. We propose that this mutation in the ANR gene is responsible for the different anthocyanin composition and high anthocyanin levels of the "black" trait in pomegranate.

 

Scientific Publication
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