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פותח על ידי קלירמאש פתרונות בע"מ -
A role for calcium-dependent protein kinases in differential CO 2 - and ABA-controlled stomatal closing and low CO 2 -induced stomatal opening in Arabidopsis
Year:
2021
Source of publication :
New Phytologist
Authors :
אזולאי, תמר
;
.
Volume :
Co-Authors:

Sebastian Schulze 
Guillaume Dubeaux 
Paulo H O Ceciliato 
Shintaro Munemasa
Maris Nuhkat 
Dmitry Yarmolinsky
Jaimee Aguilar
Renee Diaz 
Tamar Azoulay-Shemer
Leonie Steinhorst 
Jan Niklas Offenborn
Jörg Kudla 
Hannes Kollist 
Julian I Schroeder 

Facilitators :
From page:
0
To page:
0
(
Total pages:
1
)
Abstract:

Low concentrations of CO2 cause stomatal opening, whereas [CO2 ] elevation leads to stomatal closure. Classical studies have suggested a role for Ca2+ and protein phosphorylation in CO2 -induced stomatal closing. Calcium-dependent protein kinases (CPKs) and calcineurin-B-like proteins (CBLs) can sense and translate cytosolic elevation of the second messenger Ca2+ into specific phosphorylation events. However, Ca2+ -binding proteins that function in the stomatal CO2 response remain unknown. Time-resolved stomatal conductance measurements using intact plants, and guard cell patch-clamp experiments were performed. We isolated cpk quintuple mutants and analyzed stomatal movements in response to CO2 , light and abscisic acid (ABA). Interestingly, we found that cpk3/5/6/11/23 quintuple mutant plants, but not other analyzed cpk quadruple/quintuple mutants, were defective in high CO2 -induced stomatal closure and, unexpectedly, also in low CO2 -induced stomatal opening. Furthermore, K+ -uptake-channel activities were reduced in cpk3/5/6/11/23 quintuple mutants, in correlation with the stomatal opening phenotype. However, light-mediated stomatal opening remained unaffected, and ABA responses showed slowing in some experiments. By contrast, CO2 -regulated stomatal movement kinetics were not clearly affected in plasma membrane-targeted cbl1/4/5/8/9 quintuple mutant plants. Our findings describe combinatorial cpk mutants that function in CO2 control of stomatal movements and support the results of classical studies showing a role for Ca2+ in this response.

Note:
Related Files :
arabidopsis
calcium
Carbon dioxide
CO2
gas exchange
guard cells
עוד תגיות
תוכן קשור
More details
DOI :
10.1111/nph.17079
Article number:
0
Affiliations:
Database:
PubMed
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
55017
Last updated date:
02/03/2022 17:27
Creation date:
19/05/2021 13:13
Scientific Publication
A role for calcium-dependent protein kinases in differential CO 2 - and ABA-controlled stomatal closing and low CO 2 -induced stomatal opening in Arabidopsis

Sebastian Schulze 
Guillaume Dubeaux 
Paulo H O Ceciliato 
Shintaro Munemasa
Maris Nuhkat 
Dmitry Yarmolinsky
Jaimee Aguilar
Renee Diaz 
Tamar Azoulay-Shemer
Leonie Steinhorst 
Jan Niklas Offenborn
Jörg Kudla 
Hannes Kollist 
Julian I Schroeder 

Low concentrations of CO2 cause stomatal opening, whereas [CO2 ] elevation leads to stomatal closure. Classical studies have suggested a role for Ca2+ and protein phosphorylation in CO2 -induced stomatal closing. Calcium-dependent protein kinases (CPKs) and calcineurin-B-like proteins (CBLs) can sense and translate cytosolic elevation of the second messenger Ca2+ into specific phosphorylation events. However, Ca2+ -binding proteins that function in the stomatal CO2 response remain unknown. Time-resolved stomatal conductance measurements using intact plants, and guard cell patch-clamp experiments were performed. We isolated cpk quintuple mutants and analyzed stomatal movements in response to CO2 , light and abscisic acid (ABA). Interestingly, we found that cpk3/5/6/11/23 quintuple mutant plants, but not other analyzed cpk quadruple/quintuple mutants, were defective in high CO2 -induced stomatal closure and, unexpectedly, also in low CO2 -induced stomatal opening. Furthermore, K+ -uptake-channel activities were reduced in cpk3/5/6/11/23 quintuple mutants, in correlation with the stomatal opening phenotype. However, light-mediated stomatal opening remained unaffected, and ABA responses showed slowing in some experiments. By contrast, CO2 -regulated stomatal movement kinetics were not clearly affected in plasma membrane-targeted cbl1/4/5/8/9 quintuple mutant plants. Our findings describe combinatorial cpk mutants that function in CO2 control of stomatal movements and support the results of classical studies showing a role for Ca2+ in this response.

Scientific Publication
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