Strigolactones (SLs) are plant hormones that affect the development and architecture of different parts of the plant. SLs are sensed by plants via a specific intracellular and cytoplasmatic reception system. We found that SL-signaling triggers changes in F-actin and endosomal trafficking. As a result, they regulate the level of the auxin transporters PIN-FORMED (PIN) proteins in the plasma membrane (PM) of cells, and thereby, possibly, auxin efflux. The SLs-associated changes in F-actin and PIN protein localization may lead to either enhancement or depletion of PIN proteins from the PM and may underlie SLs non-cell autonomous effect.