חיפוש מתקדם

Manish Kumar Patel
Sonika Pandey
Jaykumar Patel
Avinash Mishra

Metallothioneins are cysteine-rich proteins, which play key roles in metal detoxification, intracellular ion homeostasis maintenance, and protection against intracellular oxidative damage. This study reports the characterization of SbMT-2 cloned from Salicornia brachiata concerning physiology, molecular, and photosynthesis efficiency. Overexpression of SbMT-2 conferred enhanced tolerance to Zn stress in transgenic tobacco supported by increased photosynthesis efficiency and crop quality compared to wild-type. The growth of transgenic and WT plants was comparable in control conditions; transgenic plants showed better growth than WT plants under stress (20 mM ZnSO4 for 30 days). After 30 days of stress, transgenic plants completed their life cycle with early maturation, whereas the WT plants did not and matured very late. About 35 pods with a total weight of 3.5 g per plant were measured in transgenic compared to WT (about 19 pods with total weight 2.5 g). Further, gas exchange and fluorescence measurements established that the SbMT-2 gene improved the photosynthetic efficiency of the transgenic line compared to WT plants during stress conditions. In addition, SbMT-2 might be involved in the selective translocation of Zn2+ under long-term stress condition. Microarray analysis showed that the expression of many Zn transporters, Zn-binding proteins, and Zn-associated proteins encoding genes was up-regulated 4–6 (log2) fold. However the expression of genes encoding other metal-binding proteins, metal metabolism-associated enzymes, and metal-inducible transcription factors were down-regulated. Furthermore, transcript expression analysis elucidated that overexpression of the SbMT-2 gene may regulate the expression pattern of metal transporter encoding genes under stress conditions. Thus, SbMT-2 is an important gene, which plays a positive role by detoxifying reactive oxygen species and maintaining photosynthesis efficiency under Zn stress conditions.

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תנאי שימוש
A type 2 metallothionein (SbMT-2) gene cloned from Salicornia brachiata confers enhanced Zn stress-tolerance in transgenic tobacco by transporting Zn2+ and maintaining photosynthesis efficacy
191

Manish Kumar Patel
Sonika Pandey
Jaykumar Patel
Avinash Mishra

A type 2 metallothionein (SbMT-2) gene cloned from Salicornia brachiata confers enhanced Zn stress-tolerance in transgenic tobacco by transporting Zn2+ and maintaining photosynthesis efficacy

Metallothioneins are cysteine-rich proteins, which play key roles in metal detoxification, intracellular ion homeostasis maintenance, and protection against intracellular oxidative damage. This study reports the characterization of SbMT-2 cloned from Salicornia brachiata concerning physiology, molecular, and photosynthesis efficiency. Overexpression of SbMT-2 conferred enhanced tolerance to Zn stress in transgenic tobacco supported by increased photosynthesis efficiency and crop quality compared to wild-type. The growth of transgenic and WT plants was comparable in control conditions; transgenic plants showed better growth than WT plants under stress (20 mM ZnSO4 for 30 days). After 30 days of stress, transgenic plants completed their life cycle with early maturation, whereas the WT plants did not and matured very late. About 35 pods with a total weight of 3.5 g per plant were measured in transgenic compared to WT (about 19 pods with total weight 2.5 g). Further, gas exchange and fluorescence measurements established that the SbMT-2 gene improved the photosynthetic efficiency of the transgenic line compared to WT plants during stress conditions. In addition, SbMT-2 might be involved in the selective translocation of Zn2+ under long-term stress condition. Microarray analysis showed that the expression of many Zn transporters, Zn-binding proteins, and Zn-associated proteins encoding genes was up-regulated 4–6 (log2) fold. However the expression of genes encoding other metal-binding proteins, metal metabolism-associated enzymes, and metal-inducible transcription factors were down-regulated. Furthermore, transcript expression analysis elucidated that overexpression of the SbMT-2 gene may regulate the expression pattern of metal transporter encoding genes under stress conditions. Thus, SbMT-2 is an important gene, which plays a positive role by detoxifying reactive oxygen species and maintaining photosynthesis efficiency under Zn stress conditions.

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