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פותח על ידי קלירמאש פתרונות בע"מ -
Characterization of a chickpea mutant resistant to phelipanche aegyptiaca pers. And orobanche crenata forsk
Year:
2021
Source of publication :
צמחים
Authors :
בללו, אהרון
;
.
גלילי, שמואל
;
.
דור, יבגניה
;
.
הרשנהורן, יוסף
;
.
סמירנוב, יבגני
;
.
שגב, אורית
;
.
Volume :
10(12)
Co-Authors:

Shmuel Galili

Joseph Hershenhorn

Evgeny Smirnov

Koichi Yoneyama

Xiaonan Xie

Orit Amir-Segev

Aharon Bellalou

Evgenia Dor

 

Facilitators :
From page:
0
To page:
0
(
Total pages:
1
)
Abstract:

Chickpea (Cicer arietinum L.) is a major pulse crop in Israel grown on about 3000 ha spread, from the Upper Galilee in the north to the North-Negev desert in the south. In the last few years, there has been a gradual increase in broomrape infestation in chickpea fields in all regions of Israel. Resistant chickpea cultivars would be simple and effective solution to control broomrape. Thus, to develop resistant cultivars we screened an ethyl methanesulfonate (EMS) mutant population of F01 variety (Kabuli type) for broomrape resistance. One of the mutant lines (CCD7M14) was found to be highly resistant to both Phelipanche aegyptiaca and Orobanche crenata. The resistance mechanism is based on the inability of the mutant to produce strigolactones (SLs)—stimulants of broomrape seed germination. LC/MS/MS analysis revealed the SLs orobanchol, orobanchyl acetate, and didehydroorobanchol in root exudates of the wild type, but no SLs could be detected in the root exudates of CCD7M14. Sequence analyses revealed a point mutation (G-to-A transition at nucleotide position 210) in the Carotenoid Cleavage Dioxygenase 7 (CCD7) gene that is responsible for the production of key enzymes in the biosynthesis of SLs. This nonsense mutation resulted in a CCD7 stop codon at position 70 of the protein. The influences of the CCD7M14 mutation on chickpea phenotype and chlorophyll, carotenoid, and anthocyanin content were characterized. 

Note:
Related Files :
anthocyanin
broomrape resistance
carotenoid
chickpea
chickpea phenotype
chlorophyll
Strigolactone
עוד תגיות
תוכן קשור
More details
DOI :
10.3390/plants10122552
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
57253
Last updated date:
02/03/2022 17:27
Creation date:
20/12/2021 15:55
Scientific Publication
Characterization of a chickpea mutant resistant to phelipanche aegyptiaca pers. And orobanche crenata forsk
10(12)

Shmuel Galili

Joseph Hershenhorn

Evgeny Smirnov

Koichi Yoneyama

Xiaonan Xie

Orit Amir-Segev

Aharon Bellalou

Evgenia Dor

 

Characterization of a chickpea mutant resistant to phelipanche aegyptiaca pers. And orobanche crenata forsk

Chickpea (Cicer arietinum L.) is a major pulse crop in Israel grown on about 3000 ha spread, from the Upper Galilee in the north to the North-Negev desert in the south. In the last few years, there has been a gradual increase in broomrape infestation in chickpea fields in all regions of Israel. Resistant chickpea cultivars would be simple and effective solution to control broomrape. Thus, to develop resistant cultivars we screened an ethyl methanesulfonate (EMS) mutant population of F01 variety (Kabuli type) for broomrape resistance. One of the mutant lines (CCD7M14) was found to be highly resistant to both Phelipanche aegyptiaca and Orobanche crenata. The resistance mechanism is based on the inability of the mutant to produce strigolactones (SLs)—stimulants of broomrape seed germination. LC/MS/MS analysis revealed the SLs orobanchol, orobanchyl acetate, and didehydroorobanchol in root exudates of the wild type, but no SLs could be detected in the root exudates of CCD7M14. Sequence analyses revealed a point mutation (G-to-A transition at nucleotide position 210) in the Carotenoid Cleavage Dioxygenase 7 (CCD7) gene that is responsible for the production of key enzymes in the biosynthesis of SLs. This nonsense mutation resulted in a CCD7 stop codon at position 70 of the protein. The influences of the CCD7M14 mutation on chickpea phenotype and chlorophyll, carotenoid, and anthocyanin content were characterized. 

Scientific Publication
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