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N-acetyl-β-D-glucosaminidase activity assay for monitoring insulin-dependent diabetes using Ag-porous Si SERS platform
Year:
2021
Source of publication :
Talanta
Authors :
דביר, חי
;
.
ניראלה, נרסינג
;
.
שטנברג, גיאורגי
;
.
Volume :
Co-Authors:

Narsingh R. Nirala
Jimmy Asiku
Hay Dvir
Giorgi Shtenberg

Facilitators :
From page:
0
To page:
0
(
Total pages:
1
)
Abstract:

Determination of urinary or serum N-acetyl-β-d-glucosaminidase (NAG) activity as a tissue damage indicator is widely used in diagnosis of various pathologies, including diabetic nephropathy. Early and rapid biomarker detection is an important element of medical diagnosis, facilitating prompt therapeutic decisions and prognosis evaluation. Herein, we present a modified sensing approach for a rapid and reliable NAG activity determination in complex media using surface-enhanced Raman spectroscopy (SERS). Porous silicon (PSi) Fabry-Pérot interferometers were redesigned as sensitive SERS platforms utilizing the vast inherent surface area for silver (Ag) nanoparticles embedment. Interaction of the porous nanostructures with specific NAG-enzymatic products produces an indicative spectral fingerprint proportional in magnitude to its concentration. The sensitivity of Ag–PSi SERS substrates was evaluated in complex matrices presenting sufficient limits of detection compared with other advanced assays and techniques (0.07, 0.47 and 0.50 mU mL−1 for urine, milk and plasma, respectively). The augmented optical performance revealed recovery values of 96–109%, indicating successful and selective NAG recognition in biological fluids. Finally, the potential applicability of the suggested prototype for real-life scenarios was evaluated in vivo, in a model of insulin-dependent diabetes induced in sheep. Overall, the robust data confirm the application of SERS analysis for early diagnosis of pathology and for evaluation of clinical responses to pharmacological treatments.

Note:
Related Files :
Diabetes
NAGase
Optical biosensor
Porous silicon
SERS
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/j.talanta.2021.123087
Article number:
123087
Affiliations:
Database:
סקופוס
Publication Type:
מאמר בדפוס
;
.
Language:
אנגלית
Editors' remarks:
ID:
57472
Last updated date:
02/03/2022 17:27
Creation date:
04/01/2022 19:08
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Scientific Publication
N-acetyl-β-D-glucosaminidase activity assay for monitoring insulin-dependent diabetes using Ag-porous Si SERS platform

Narsingh R. Nirala
Jimmy Asiku
Hay Dvir
Giorgi Shtenberg

N-acetyl-β-D-glucosaminidase activity assay for monitoring insulin-dependent diabetes using Ag-porous Si SERS platform .

Determination of urinary or serum N-acetyl-β-d-glucosaminidase (NAG) activity as a tissue damage indicator is widely used in diagnosis of various pathologies, including diabetic nephropathy. Early and rapid biomarker detection is an important element of medical diagnosis, facilitating prompt therapeutic decisions and prognosis evaluation. Herein, we present a modified sensing approach for a rapid and reliable NAG activity determination in complex media using surface-enhanced Raman spectroscopy (SERS). Porous silicon (PSi) Fabry-Pérot interferometers were redesigned as sensitive SERS platforms utilizing the vast inherent surface area for silver (Ag) nanoparticles embedment. Interaction of the porous nanostructures with specific NAG-enzymatic products produces an indicative spectral fingerprint proportional in magnitude to its concentration. The sensitivity of Ag–PSi SERS substrates was evaluated in complex matrices presenting sufficient limits of detection compared with other advanced assays and techniques (0.07, 0.47 and 0.50 mU mL−1 for urine, milk and plasma, respectively). The augmented optical performance revealed recovery values of 96–109%, indicating successful and selective NAG recognition in biological fluids. Finally, the potential applicability of the suggested prototype for real-life scenarios was evaluated in vivo, in a model of insulin-dependent diabetes induced in sheep. Overall, the robust data confirm the application of SERS analysis for early diagnosis of pathology and for evaluation of clinical responses to pharmacological treatments.

Scientific Publication
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