תפריט נגישות
ניגודיות עדינהניגודיות גבוהה
מונוכרום
הדגשת קישורים
חסימת אנימציה
פונט קריא
סגור
איפוס הגדרות נגישות
להורדת מודול נגישות חינם תפריט נגישותניגודיות עדינהניגודיות גבוההמונוכרוםהדגשת קישוריםחסימת אנימציהפונט קריאסגוראיפוס הגדרות נגישותלהורדת מודול נגישות חינםQili Liu
Rui Zhang
Huali Xue
Yang Bi
Lan Li
Qianqian Zhang
Carelle Jimdjio Kouasseu
Mina Nan
Dov Prusky
Fusarium sulphureum is a major plant pathogenic fungus that can cause dry rot of potato. Ozone, a strong oxidant, is generally regarded as a safe (GRAS) substance to widely use to manage postharvest decay in fruit and vegetables. The effect of ozone treatment on the mycelia growth, spore germination, cell membrane integrity and morphological and ultrastructural alteration, as well as intracellular reactive species oxygen (ROS) generation of F. sulphureum in vitro, and dry rot development and diacetoxyscirpenol (DAS) in vivo was conducted. Results demonstrated 2 mg L−1 ozone treatment effectively inhibited mycelia growth and spore germination, disturbed the cell membrane integrity of F. sulphureum in vitro due to intracellular ROS production, moreover, ozone lead to morphological alteration such as obvious shriveled and sunken on the surface, even some broken fragment was observed; transmission electron microscopy observation suggested some organelles such as, cytoplasm mitochondria and cell wall were suffered serious destruction. The inhibitory efficacy on F. sulphureum spore, as a consequently, lead to the suppressing the dry rot and DAS accumulation in vivo.
Qili Liu
Rui Zhang
Huali Xue
Yang Bi
Lan Li
Qianqian Zhang
Carelle Jimdjio Kouasseu
Mina Nan
Dov Prusky
Fusarium sulphureum is a major plant pathogenic fungus that can cause dry rot of potato. Ozone, a strong oxidant, is generally regarded as a safe (GRAS) substance to widely use to manage postharvest decay in fruit and vegetables. The effect of ozone treatment on the mycelia growth, spore germination, cell membrane integrity and morphological and ultrastructural alteration, as well as intracellular reactive species oxygen (ROS) generation of F. sulphureum in vitro, and dry rot development and diacetoxyscirpenol (DAS) in vivo was conducted. Results demonstrated 2 mg L−1 ozone treatment effectively inhibited mycelia growth and spore germination, disturbed the cell membrane integrity of F. sulphureum in vitro due to intracellular ROS production, moreover, ozone lead to morphological alteration such as obvious shriveled and sunken on the surface, even some broken fragment was observed; transmission electron microscopy observation suggested some organelles such as, cytoplasm mitochondria and cell wall were suffered serious destruction. The inhibitory efficacy on F. sulphureum spore, as a consequently, lead to the suppressing the dry rot and DAS accumulation in vivo.
