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פותח על ידי קלירמאש פתרונות בע"מ -
Insights into gene manipulation techniques for Acari functional genomics
Year:
2022
Authors :
נפוסי, ביאטריס
;
.
סורוקר, ויקטוריה
;
.
פינס, גור
;
.
Volume :
143
Co-Authors:

Beatrice T Nganso 
Gur Pines 
Victoria Soroker 

Facilitators :
From page:
0
To page:
0
(
Total pages:
1
)
Abstract:

Functional genomics is an essential tool for elucidating the structure and function of genes in any living organism. Here, we review the use of different gene manipulation techniques in functional genomics of Acari (mites and ticks). Some of these Acari species inflict severe economic losses to managed crops and health problems to humans, wild and domestic animals, but many also provide important ecosystem services worldwide. Currently, RNA interference (RNAi) is the leading gene expression manipulation tool followed by gene editing via the bacterial type II Clustered Regularly Interspaced Short Palindromic Repeats and associated protein 9 system (CRISPR-Cas9). Whilst RNAi, via siRNA, does not always lead to expected outcomes, the exploitations of the CRISPR systems in Acari are still in their infancy and are limited only to CRISP/Cas9 to date. In this review, we discuss the advantages and disadvantages of RNAi and CRISPR-Cas9 and the technical challenges associated with their exploitations. We also compare the biochemical machinery of RNAi and CRISPR-Cas9 technologies. We highlight some potential solutions for experimental optimization of each mechanism in gene function studies. The potential benefits of adopting various CRISPR-Cas9 systems for expanding on functional genomics experiments in Acari are also discussed.

Note:
Related Files :
Chelicerata
CRISPR
Gene editing  
gene silencing
RNAi
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/j.ibmb.2021.103705
Article number:
103705
Affiliations:
Database:
PubMed
Publication Type:
סקירה
;
.
Language:
אנגלית
Editors' remarks:
ID:
57983
Last updated date:
02/03/2022 17:27
Creation date:
20/02/2022 12:57
Scientific Publication
Insights into gene manipulation techniques for Acari functional genomics
143

Beatrice T Nganso 
Gur Pines 
Victoria Soroker 

Insights into gene manipulation techniques for Acari functional genomics

Functional genomics is an essential tool for elucidating the structure and function of genes in any living organism. Here, we review the use of different gene manipulation techniques in functional genomics of Acari (mites and ticks). Some of these Acari species inflict severe economic losses to managed crops and health problems to humans, wild and domestic animals, but many also provide important ecosystem services worldwide. Currently, RNA interference (RNAi) is the leading gene expression manipulation tool followed by gene editing via the bacterial type II Clustered Regularly Interspaced Short Palindromic Repeats and associated protein 9 system (CRISPR-Cas9). Whilst RNAi, via siRNA, does not always lead to expected outcomes, the exploitations of the CRISPR systems in Acari are still in their infancy and are limited only to CRISP/Cas9 to date. In this review, we discuss the advantages and disadvantages of RNAi and CRISPR-Cas9 and the technical challenges associated with their exploitations. We also compare the biochemical machinery of RNAi and CRISPR-Cas9 technologies. We highlight some potential solutions for experimental optimization of each mechanism in gene function studies. The potential benefits of adopting various CRISPR-Cas9 systems for expanding on functional genomics experiments in Acari are also discussed.

Scientific Publication
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