נגישות
menu      
חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Genome-wide recombination map construction from single sperm sequencing in cattle
Year:
2022
Source of publication :
BMC Genomics
Authors :
סרוסי, אייל
;
.
Volume :
23
Co-Authors:

Liu Yang 
Yahui Gao 
Mingxun Li
Ki-Eun Park
Shuli Liu
Xiaolong Kang
Mei Liu 
Adam Oswalt
Lingzhao Fang
Bhanu P Telugu
Charles G Sattler
Cong-Jun Li 
John B Cole
Lingyang Xu
Lv Yang 
Yang Zhou
Li Li 
Hongping Zhang 
Benjamin D Rosen
Curtis P Van Tassell 
Li Ma
George E Liu

Facilitators :
From page:
1
To page:
9
(
Total pages:
9
)
Abstract:

Background: Meiotic recombination is one of the important phenomena contributing to gamete genome diversity. However, except for human and a few model organisms, it is not well studied in livestock, including cattle.

Results: To investigate their distributions in the cattle sperm genome, we sequenced 143 single sperms from two Holstein bulls. We mapped meiotic recombination events at high resolution based on phased heterozygous single nucleotide polymorphism (SNP). In the absence of evolutionary selection pressure in fertilization and survival, recombination events in sperm are enriched near distal chromosomal ends, revealing that such a pattern is intrinsic to the molecular mechanism of meiosis. Furthermore, we further validated these findings in single sperms with results derived from sequencing its family trio of diploid genomes and our previous studies of recombination in cattle.

Conclusions: To our knowledge, this is the first large-scale single sperm whole-genome sequencing effort in livestock, which provided useful information for future studies of recombination, genome instability, and male infertility.

Note:
Related Files :
cattle
recombination
Sequencing
עוד תגיות
תוכן קשור
More details
DOI :
10.1186/s12864-022-08415-w
Article number:
181
Affiliations:
Database:
PubMed
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
58242
Last updated date:
16/03/2022 16:23
Creation date:
16/03/2022 12:44
You may also be interested in
Scientific Publication
Genome-wide recombination map construction from single sperm sequencing in cattle
23

Liu Yang 
Yahui Gao 
Mingxun Li
Ki-Eun Park
Shuli Liu
Xiaolong Kang
Mei Liu 
Adam Oswalt
Lingzhao Fang
Bhanu P Telugu
Charles G Sattler
Cong-Jun Li 
John B Cole
Lingyang Xu
Lv Yang 
Yang Zhou
Li Li 
Hongping Zhang 
Benjamin D Rosen
Curtis P Van Tassell 
Li Ma
George E Liu

Genome-wide recombination map construction from single sperm sequencing in cattle

Background: Meiotic recombination is one of the important phenomena contributing to gamete genome diversity. However, except for human and a few model organisms, it is not well studied in livestock, including cattle.

Results: To investigate their distributions in the cattle sperm genome, we sequenced 143 single sperms from two Holstein bulls. We mapped meiotic recombination events at high resolution based on phased heterozygous single nucleotide polymorphism (SNP). In the absence of evolutionary selection pressure in fertilization and survival, recombination events in sperm are enriched near distal chromosomal ends, revealing that such a pattern is intrinsic to the molecular mechanism of meiosis. Furthermore, we further validated these findings in single sperms with results derived from sequencing its family trio of diploid genomes and our previous studies of recombination in cattle.

Conclusions: To our knowledge, this is the first large-scale single sperm whole-genome sequencing effort in livestock, which provided useful information for future studies of recombination, genome instability, and male infertility.

Scientific Publication
You may also be interested in