Zhicheng Li , Xiaoqin Xu , Sulin Xue, Di Gong , Bin Wang , Xiaoyuan Zheng , Pengdong Xie , Yang Bi 

As an important elicitor, chitosan could activate the synthesis of lignin in many plants. However, no report is available on whether preharvest chitosan sprays affects the synthesis and deposition of lignin at wounds of harvested muskmelons. In the present study, the plants and fruit of muskmelons were multiple sprayed with 0.1% chitosan during fruit development. Here, we found that chitosan sprays increased the activities of 4-coumaric acid-coenzyme A ligase, cinnamyl-CoA reductase and cinnamyl alcohol dehydrogenase, and elevated the levels of p-coumaryl alcohol, coniferyl alcohol, sinapyl alcohol and lignin at wounds. Chitosan sprays enhanced H₂O₂ level and peroxidase activity, and accelerated the deposition of lignin at wounds. Moreover, chitosan sprays resulted in a higher hardness and lower resilience, springiness and cohesiveness of the healing tissues. Taken together, preharvest chitosan sprays accelerated the deposition of lignin at wounds of muskmelons by activating lignin metabolism, and increasing H₂O₂ content and peroxidase activity.