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פותח על ידי קלירמאש פתרונות בע"מ -
DNAzyme-based biosensor for sub ppb lead ions detection using porous silicon Fabry-Pérot interferometer
Year:
2022
Source of publication :
Sensors and Actuators B: Chemical
Authors :
שמש, משה
;
.
Volume :
362
Co-Authors:

D. Nanda Kumar
Shirly Reingewirtz
Moshe Shemesh
Ran Suckeveriene
Giorgi Shtenberg

Facilitators :
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0
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Total pages:
1
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Abstract:

Herein, we present an optical sensing approach based on nanostructured porous silicon (PSi) Fabry-Pérot interferometers for real-time monitoring of lead (Pb2+) contaminants using DNAzyme, a synthetic DNA sequence with a functional catalytic region. The latter cleaved the complementary DNA/RNA substrate strand in the presence of Pb2+, which resulted in a decrease in the average refractive index of the total porous void. The interfacial optical contrast was enhanced by coupling silica nanoparticles to the complementary strand, thus improving the interferometer’s inherent performance. The catalytic activity of the DNAzyme-modified platform was monitored in real-time using reflective interferometric Fourier transform spectroscopy. The optimized conditions offered means for accurate, rapid (~20 min) and precise detection of Pb2+ in the range of 0.1–25 ppb with a detection limit of 0.49 ppb (2.4 nM). Sufficient selectivity and specificity were attained against other commonly known metallic pollutants in water sources. Finally, the practical applicability of the biosensor’s performance was demonstrated using real samples, such as ground, irrigation, tap water and even plasma, while depicting satisfactory recovery values of 94–103% in compliance with the ICP-MS approach. Overall, the developed sensing platform coupled to optical signal amplification can be easily redesigned for targeting other metal pollutants posing risks to water sources and the environment.

Note:
Related Files :
DNAzyme
Fabry-Pérot
Heavy metals
Interferometer
Lead ions
Porous silicon
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/j.snb.2022.131761
Article number:
131761
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
58556
Last updated date:
11/04/2022 17:23
Creation date:
11/04/2022 17:09
Scientific Publication
DNAzyme-based biosensor for sub ppb lead ions detection using porous silicon Fabry-Pérot interferometer
362

D. Nanda Kumar
Shirly Reingewirtz
Moshe Shemesh
Ran Suckeveriene
Giorgi Shtenberg

DNAzyme-based biosensor for sub ppb lead ions detection using porous silicon Fabry-Pérot interferometer

Herein, we present an optical sensing approach based on nanostructured porous silicon (PSi) Fabry-Pérot interferometers for real-time monitoring of lead (Pb2+) contaminants using DNAzyme, a synthetic DNA sequence with a functional catalytic region. The latter cleaved the complementary DNA/RNA substrate strand in the presence of Pb2+, which resulted in a decrease in the average refractive index of the total porous void. The interfacial optical contrast was enhanced by coupling silica nanoparticles to the complementary strand, thus improving the interferometer’s inherent performance. The catalytic activity of the DNAzyme-modified platform was monitored in real-time using reflective interferometric Fourier transform spectroscopy. The optimized conditions offered means for accurate, rapid (~20 min) and precise detection of Pb2+ in the range of 0.1–25 ppb with a detection limit of 0.49 ppb (2.4 nM). Sufficient selectivity and specificity were attained against other commonly known metallic pollutants in water sources. Finally, the practical applicability of the biosensor’s performance was demonstrated using real samples, such as ground, irrigation, tap water and even plasma, while depicting satisfactory recovery values of 94–103% in compliance with the ICP-MS approach. Overall, the developed sensing platform coupled to optical signal amplification can be easily redesigned for targeting other metal pollutants posing risks to water sources and the environment.

Scientific Publication
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