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פותח על ידי קלירמאש פתרונות בע"מ -
Identification of positive and negative regulators of antiviral RNA interference in Arabidopsis thaliana
Year:
2022
Source of publication :
Nature Communications
Authors :
גל-און, עמית
;
.
Volume :
Co-Authors:

Si Liu

Meijuan Chen
Ruidong Li
Wan-Xiang Li
Amit Gal-On
Zhenyu Jia
Shou-Wei Ding  

Facilitators :
From page:
0
To page:
0
(
Total pages:
1
)
Abstract:

Virus-host coevolution often drives virus immune escape. However, it remains unknown whether natural variations of plant virus resistance are enriched in genes of RNA interference (RNAi) pathway known to confer essential antiviral defense in plants. Here, we report two genome-wide association study screens to interrogate natural variation among wild-collected Arabidopsis thaliana accessions in quantitative resistance to the endemic cucumber mosaic virus (CMV). We demonstrate that the highest-ranked gene significantly associated with resistance from both screens acts to regulate antiviral RNAi in ecotype Columbia-0. One gene, corresponding to Reduced Dormancy 5 (RDO5), enhances resistance by promoting amplification of the virus-derived small interfering RNAs (vsiRNAs). Interestingly, the second gene, designated Antiviral RNAi Regulator 1 (VIR1), dampens antiviral RNAi so its genetic inactivation by CRISPR/Cas9 editing enhances both vsiRNA production and CMV resistance. Our findings identify positive and negative regulators of the antiviral RNAi defense that may play important roles in virus-host coevolution.

Note:
Related Files :
Antiviral
Arabidopsis thaliana
RNA interference (RNAi)
עוד תגיות
תוכן קשור
More details
DOI :
10.1038/s41467-022-30771-0
Article number:
0
Affiliations:
Database:
PubMed
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
59308
Last updated date:
08/06/2022 14:02
Creation date:
08/06/2022 14:02
Scientific Publication
Identification of positive and negative regulators of antiviral RNA interference in Arabidopsis thaliana

Si Liu

Meijuan Chen
Ruidong Li
Wan-Xiang Li
Amit Gal-On
Zhenyu Jia
Shou-Wei Ding  

Identification of positive and negative regulators of antiviral RNA interference in Arabidopsis thaliana .

Virus-host coevolution often drives virus immune escape. However, it remains unknown whether natural variations of plant virus resistance are enriched in genes of RNA interference (RNAi) pathway known to confer essential antiviral defense in plants. Here, we report two genome-wide association study screens to interrogate natural variation among wild-collected Arabidopsis thaliana accessions in quantitative resistance to the endemic cucumber mosaic virus (CMV). We demonstrate that the highest-ranked gene significantly associated with resistance from both screens acts to regulate antiviral RNAi in ecotype Columbia-0. One gene, corresponding to Reduced Dormancy 5 (RDO5), enhances resistance by promoting amplification of the virus-derived small interfering RNAs (vsiRNAs). Interestingly, the second gene, designated Antiviral RNAi Regulator 1 (VIR1), dampens antiviral RNAi so its genetic inactivation by CRISPR/Cas9 editing enhances both vsiRNA production and CMV resistance. Our findings identify positive and negative regulators of the antiviral RNAi defense that may play important roles in virus-host coevolution.

Scientific Publication
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