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פותח על ידי קלירמאש פתרונות בע"מ -
Embryonic heat conditioning in chicks induces transgenerational heat/immunological resilience via methylation on regulatory elements
Year:
2022
Source of publication :
FASEB Journal
Authors :
דרוין, שלי
;
.
הראון, עמית
;
.
מאירי, נעם
;
.
קיסיליוק, טטיאנה
;
.
רוזנברג, טלי
;
.
שינדר, דמיטרי
;
.
Volume :
Co-Authors:

Tali Rosenberg
Asaf Marco
Tatiana Kisliouk 
Amit Haron
Dmitry Shinder 
Shelly Druyan
Noam Meiri  

Facilitators :
From page:
0
To page:
0
(
Total pages:
1
)
Abstract:

The question of whether behavioral traits are heritable is under debate. An obstacle in demonstrating transgenerational inheritance in mammals originates from the maternal environment's effect on offspring phenotype. Here, we used in ovo embryonic heat conditioning (EHC) of first-generation chicks, demonstrating heredity of both heat and immunological resilience, confirmed by a reduced fibril response in their untreated offspring to either heat or LPS challenge. Concordantly, transcriptome analysis confirmed that EHC induces changes in gene expression in the anterior preoptic hypothalamus (APH) that contribute to these phenotypes in the offspring. To study the association between epigenetic mechanisms and trait heritability, DNA-methylation patterns in the APH of offspring of control versus EHC fathers were evaluated. Genome-wide analysis revealed thousands of differentially methylated sites (DMSs), which were highly enriched in enhancers and CCCTC-binding factor (CTCF) sites. Overlap analysis revealed 110 differentially expressed genes that were associated with altered methylation, predominantly on enhancers. Gene-ontology analysis shows pathways associated with immune response, chaperone-mediated protein folding, and stress response. For the proof of concept, we focused on HSP25 and SOCS3, modulators of heat and immune responses, respectively. Chromosome conformational capture (3C) assay identified interactions between their promoters and methylated enhancers, with the strongest frequency on CTCF binding sites. Furthermore, gene expression corresponded with the differential methylation patterns, and presented increased CTCF binding in both hyper- and hypomethylated DMSs. Collectively, we demonstrate that EHC induces transgenerational thermal and immunological resilience traits. We propose that one of the mechanisms underlying inheritance depends on three-dimensional (3D) chromatin reorganization.

Note:
Related Files :
Cross-tolerance
embryonic heat conditioning
epigenetics
Heredity
hypothalamus
stress
עוד תגיות
תוכן קשור
More details
DOI :
10.1096/fj.202101948R
Article number:
0
Affiliations:
Database:
PubMed
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
60002
Last updated date:
28/06/2022 15:22
Creation date:
28/06/2022 15:22
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Scientific Publication
Embryonic heat conditioning in chicks induces transgenerational heat/immunological resilience via methylation on regulatory elements

Tali Rosenberg
Asaf Marco
Tatiana Kisliouk 
Amit Haron
Dmitry Shinder 
Shelly Druyan
Noam Meiri  

Embryonic heat conditioning in chicks induces transgenerational heat/immunological resilience via methylation on regulatory elements .

The question of whether behavioral traits are heritable is under debate. An obstacle in demonstrating transgenerational inheritance in mammals originates from the maternal environment's effect on offspring phenotype. Here, we used in ovo embryonic heat conditioning (EHC) of first-generation chicks, demonstrating heredity of both heat and immunological resilience, confirmed by a reduced fibril response in their untreated offspring to either heat or LPS challenge. Concordantly, transcriptome analysis confirmed that EHC induces changes in gene expression in the anterior preoptic hypothalamus (APH) that contribute to these phenotypes in the offspring. To study the association between epigenetic mechanisms and trait heritability, DNA-methylation patterns in the APH of offspring of control versus EHC fathers were evaluated. Genome-wide analysis revealed thousands of differentially methylated sites (DMSs), which were highly enriched in enhancers and CCCTC-binding factor (CTCF) sites. Overlap analysis revealed 110 differentially expressed genes that were associated with altered methylation, predominantly on enhancers. Gene-ontology analysis shows pathways associated with immune response, chaperone-mediated protein folding, and stress response. For the proof of concept, we focused on HSP25 and SOCS3, modulators of heat and immune responses, respectively. Chromosome conformational capture (3C) assay identified interactions between their promoters and methylated enhancers, with the strongest frequency on CTCF binding sites. Furthermore, gene expression corresponded with the differential methylation patterns, and presented increased CTCF binding in both hyper- and hypomethylated DMSs. Collectively, we demonstrate that EHC induces transgenerational thermal and immunological resilience traits. We propose that one of the mechanisms underlying inheritance depends on three-dimensional (3D) chromatin reorganization.

Scientific Publication
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