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פותח על ידי קלירמאש פתרונות בע"מ -
Genome-based high-resolution mapping of fusarium wilt resistance in sweet basil
Year:
2022
Source of publication :
Plant Science
Authors :
גונדה, איתי
;
.
דודאי, נתיב
;
.
דורון-פייגנבאום, עדי
;
.
Volume :
Co-Authors:

Itay Gonda 
Renana Milavski
Chen Adler 
Mohamad Abu-Abied 
Ofir Tal
 Adi Faigenboim
David Chaimovitsh 
Nativ Dudai  

Facilitators :
From page:
0
To page:
0
(
Total pages:
1
)
Abstract:

Fusarium wilt of basil is a disease of sweet basil (Ocimum basilicum L.) plants caused by the fungus Fusarium oxysporum f. sp. basilici (FOB). Although resistant cultivars were released > 20 years ago, the underlying mechanism and the genes controlling the resistance remain unknown. We used genetic mapping to elucidate FOB resistance in an F2 population derived from a cross between resistant and susceptible cultivars. We performed genotyping by sequencing of 173 offspring and aligning the data to the sweet basil reference genome. In total, 23,411 polymorphic sites were detected, and a single quantitative trait locus (QTL) for FOB resistance was found. The confidence interval was < 600 kbp, harboring only 60 genes, including a cluster of putative disease-resistance genes. Based on homology to a fusarium resistance protein from wild tomato, we also investigated a candidate resistance gene that encodes a transmembrane leucine-rich repeat - receptor-like kinase - ubiquitin-like protease (LRR-RLK-ULP). Sequence analysis of that gene in the susceptible parent vs. the resistant parent revealed multiple indels, including an insertion of 20 amino acids next to the transmembrane domain, which might alter its functionality. Our findings suggest that this LRR-RLK-ULP might be responsible for FOB resistance in sweet basil and demonstrate the usefulness of the recently sequenced basil genome for QTL mapping and gene mining.

Note:
Related Files :
Fusarium wilt
QTL mapping
Sweet basil
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/j.plantsci.2022.111316
Article number:
0
Affiliations:
Database:
PubMed
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
60004
Last updated date:
28/06/2022 17:49
Creation date:
28/06/2022 17:49
Scientific Publication
Genome-based high-resolution mapping of fusarium wilt resistance in sweet basil

Itay Gonda 
Renana Milavski
Chen Adler 
Mohamad Abu-Abied 
Ofir Tal
 Adi Faigenboim
David Chaimovitsh 
Nativ Dudai  

Genome-based high-resolution mapping of fusarium wilt resistance in sweet basil .

Fusarium wilt of basil is a disease of sweet basil (Ocimum basilicum L.) plants caused by the fungus Fusarium oxysporum f. sp. basilici (FOB). Although resistant cultivars were released > 20 years ago, the underlying mechanism and the genes controlling the resistance remain unknown. We used genetic mapping to elucidate FOB resistance in an F2 population derived from a cross between resistant and susceptible cultivars. We performed genotyping by sequencing of 173 offspring and aligning the data to the sweet basil reference genome. In total, 23,411 polymorphic sites were detected, and a single quantitative trait locus (QTL) for FOB resistance was found. The confidence interval was < 600 kbp, harboring only 60 genes, including a cluster of putative disease-resistance genes. Based on homology to a fusarium resistance protein from wild tomato, we also investigated a candidate resistance gene that encodes a transmembrane leucine-rich repeat - receptor-like kinase - ubiquitin-like protease (LRR-RLK-ULP). Sequence analysis of that gene in the susceptible parent vs. the resistant parent revealed multiple indels, including an insertion of 20 amino acids next to the transmembrane domain, which might alter its functionality. Our findings suggest that this LRR-RLK-ULP might be responsible for FOB resistance in sweet basil and demonstrate the usefulness of the recently sequenced basil genome for QTL mapping and gene mining.

Scientific Publication
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