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פותח על ידי קלירמאש פתרונות בע"מ -
Platform for Active Vaccine Formulation Using a Two-Mode Enhancement Mechanism of Epitope Presentation by Pickering Emulsion
Year:
2022
Source of publication :
ACS Applied Bio Materials
Authors :
אננט מאני, קרטיק
;
.
דומברובסקי, אביב
;
.
יעקב, נוגה
;
.
לוריא, נטע
;
.
לכמן, עודד
;
.
מולד, אורי
;
.
מחרז, גיא
;
.
סמית', אלישבע
;
.
Volume :
Co-Authors:

Guy Mechrez 
Karthik Ananth Mani 
Abhijit Saha
Oded Lachman 
Neta Luria
 Ori Molad
Liliya Kotliarevski
Einat Zelinger
Elisheva Smith 
Noga Yaakov
Dalia Shabashov Stone 
Meital Reches
Aviv Dombrovsky 

Facilitators :
From page:
0
To page:
0
(
Total pages:
1
)
Abstract:

The efficiency of epitope-based vaccination (subunit vaccines) is tightly correlated with heterogeneity and the high density of epitope presentation, which maximizes the potential antigenic determinants. Here, we developed a two-mode platform for intensifying the epitope presentation of subunit vaccines. The two-mode epitope presentation enhancement includes a covalent attachment of high concentrations of SARS-CoV-2-S1 peptide epitope to the surface of virus-like-particles (VLPs) and the subsequent assembly of VLP/epitope conjugates on the oil droplet surface at an oil/water interface of an emulsion as Pickering stabilizers. The resultant emulsions were stable for weeks in ambient conditions, and our platform was challenged using the epitope of the SARS-CoV-2-S1 peptide that served as a model epitope in this study. In vivo assays showed that the αSARS-CoV-2-S1 immunoglobulin G (IgG) titers of the studied mouse antisera, developed against the SARS-CoV-2-S1 peptide under different epitope preparation conditions, showed an order of magnitude higher IgG titers in the studied VLP-based emulsions than epitopes dissolved in water and epitopes administered with an adjuvant, thereby confirming the efficacy of the formulation. This VLP-based Pickering emulsion platform is a fully synthetic approach that can be readily applied for vaccine development to a wide range of pathogens.

Note:
Related Files :
epitope-based vaccination
Pickering emulsions
plant virus
subunit vaccines
virus-like-particles
עוד תגיות
תוכן קשור
More details
DOI :
10.1021/acsabm.2c00410
Article number:
0
Affiliations:
Database:
PubMed
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
61030
Last updated date:
07/08/2022 22:45
Creation date:
07/08/2022 22:44
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Scientific Publication
Platform for Active Vaccine Formulation Using a Two-Mode Enhancement Mechanism of Epitope Presentation by Pickering Emulsion

Guy Mechrez 
Karthik Ananth Mani 
Abhijit Saha
Oded Lachman 
Neta Luria
 Ori Molad
Liliya Kotliarevski
Einat Zelinger
Elisheva Smith 
Noga Yaakov
Dalia Shabashov Stone 
Meital Reches
Aviv Dombrovsky 

Platform for Active Vaccine Formulation Using a Two-Mode Enhancement Mechanism of Epitope Presentation by Pickering Emulsion

The efficiency of epitope-based vaccination (subunit vaccines) is tightly correlated with heterogeneity and the high density of epitope presentation, which maximizes the potential antigenic determinants. Here, we developed a two-mode platform for intensifying the epitope presentation of subunit vaccines. The two-mode epitope presentation enhancement includes a covalent attachment of high concentrations of SARS-CoV-2-S1 peptide epitope to the surface of virus-like-particles (VLPs) and the subsequent assembly of VLP/epitope conjugates on the oil droplet surface at an oil/water interface of an emulsion as Pickering stabilizers. The resultant emulsions were stable for weeks in ambient conditions, and our platform was challenged using the epitope of the SARS-CoV-2-S1 peptide that served as a model epitope in this study. In vivo assays showed that the αSARS-CoV-2-S1 immunoglobulin G (IgG) titers of the studied mouse antisera, developed against the SARS-CoV-2-S1 peptide under different epitope preparation conditions, showed an order of magnitude higher IgG titers in the studied VLP-based emulsions than epitopes dissolved in water and epitopes administered with an adjuvant, thereby confirming the efficacy of the formulation. This VLP-based Pickering emulsion platform is a fully synthetic approach that can be readily applied for vaccine development to a wide range of pathogens.

Scientific Publication
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