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פותח על ידי קלירמאש פתרונות בע"מ -
Porous silicon Fabry-Pérot interferometer designed for sensitive detection of aflatoxin B1 in field crops
Year:
2023
Source of publication :
Food Chemistry
Authors :
סיונוב, אדוארד
;
.
שטנברג, גיאורגי
;
.
Volume :
405
Co-Authors:

D. Nanda Kumar
Ilana Freidman
Edward Sionov
Giorgi Shtenberg

Facilitators :
From page:
0
To page:
0
(
Total pages:
1
)
Abstract:

Herein, a label-free sensing platform was designed for accurate, rapid and selective detection of aflatoxin B1 (AFB1), a potent mutagenic and carcinogenic substance in food and feedstuff. Minute AFB1 residues were assessed by competitive immunoassay facilitated on porous silicon Fabry-Pérot interferometer. The immunocomplex formation was biochemically amplified by enzymatic reaction products infiltrating the porous void and alternating the reflectivity spectra in correlation to the AFB1 content. The optical output presented high sensitivity toward target analyte detection in simulated conditions, as low as 0.03 ppb within the dynamic range of 0.01–10 ppb. The selectivity and specificity of the developed sensing platform were cross-validated versus commonly known interfering mycotoxins without compromising its performance values. Finally, the efficiency and the accuracy of the system were demonstrated in three matrices (maize, peanut and wheat) while demonstrating acceptable recovery values of 94–101 %, in compliance with the competitive ELISA standard assay and HPLC.

Note:
Related Files :
Aflatoxin B1
Fabry-Pérot interferometer
Foodstuff contaminants
Porous silicon
Signal amplifications
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/j.foodchem.2022.134980
Article number:
134980
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
62534
Last updated date:
27/11/2022 15:58
Creation date:
27/11/2022 15:57
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Scientific Publication
Porous silicon Fabry-Pérot interferometer designed for sensitive detection of aflatoxin B1 in field crops
405

D. Nanda Kumar
Ilana Freidman
Edward Sionov
Giorgi Shtenberg

Porous silicon Fabry-Pérot interferometer designed for sensitive detection of aflatoxin B1 in field crops

Herein, a label-free sensing platform was designed for accurate, rapid and selective detection of aflatoxin B1 (AFB1), a potent mutagenic and carcinogenic substance in food and feedstuff. Minute AFB1 residues were assessed by competitive immunoassay facilitated on porous silicon Fabry-Pérot interferometer. The immunocomplex formation was biochemically amplified by enzymatic reaction products infiltrating the porous void and alternating the reflectivity spectra in correlation to the AFB1 content. The optical output presented high sensitivity toward target analyte detection in simulated conditions, as low as 0.03 ppb within the dynamic range of 0.01–10 ppb. The selectivity and specificity of the developed sensing platform were cross-validated versus commonly known interfering mycotoxins without compromising its performance values. Finally, the efficiency and the accuracy of the system were demonstrated in three matrices (maize, peanut and wheat) while demonstrating acceptable recovery values of 94–101 %, in compliance with the competitive ELISA standard assay and HPLC.

Scientific Publication
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