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פותח על ידי קלירמאש פתרונות בע"מ -
Fruit microbiome: A powerful tool to study the epidemiology of dry lenticel rot and white haze – Emerging postharvest diseases of apple
Year:
2023
Source of publication :
Postharvest Biology and Technology
Authors :
דרובי, סמיר
;
.
Volume :
196
Co-Authors:

Marco Garello
Edoardo Piombo
Simona Prencipe
Giada Schiavon
Lorenzo Berra
Michael Wisniewski
Samir Droby
Davide Spadaro

Facilitators :
From page:
0
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0
(
Total pages:
1
)
Abstract:

With the introduction of new apple varieties, emerging diseases have been recorded including dry lenticel rot and white haze. Ramularia mali has been identified as the causal agent of dry lenticel rot, whereas species of GolubeviaTilletiopsis and Entyloma have been associated to white haze, but the epidemiology of these pathogens remains unclear. In the present study, we measured fruit disease incidence and quality parameters, and we used metabarcoding to characterize both epiphytic and endophytic microbial communities of apple fruit of two commercial cultivars, ‘Opal’ and ‘Ambrosia’, across six time points from early fruit development up to the end of shelf life. R. mali first develops in both cultivars as an endophyte at BBCH (Biologische Bundesanstalt, Bundessortenamt and CHemical industry) phenological phase 73 (10–11% relative abundance), BBCH 77 (26–33% relative abundance) and BBCH 81 (1–7% relative abundance), then it appears as an epiphyte from BBCH 87 onward (1–2% relative abundance), when symptoms start to be visible. This was confirmed in endophytic samples through qPCR specific for R. mali. Among the genera associated to white haze, Golubevia was the most abundant epiphyte (2–4%) from BBCH 81 to the end of shelf life. Alpha and beta diversity analyses unveiled the presence of significant difference both in richness and composition among different tissue, time points and cultivars. In conclusion, the study helps to explain the epidemiology of white haze and dry lenticel rot, and to design a targeted crop protection strategy, reinforcing the hypothesis that fruit metabarcoding could be a valuable tool for assessment and prediction of postharvest diseases, before symptoms occurrence in fruit.

Note:
Related Files :
Apple
Dry lenticel rot
metabarcoding
microbiome
Postharvest disease
White haze
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/j.postharvbio.2022.112163
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
62634
Last updated date:
06/12/2022 17:31
Creation date:
06/12/2022 17:31
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Scientific Publication
Fruit microbiome: A powerful tool to study the epidemiology of dry lenticel rot and white haze – Emerging postharvest diseases of apple
196

Marco Garello
Edoardo Piombo
Simona Prencipe
Giada Schiavon
Lorenzo Berra
Michael Wisniewski
Samir Droby
Davide Spadaro

Fruit microbiome: A powerful tool to study the epidemiology of dry lenticel rot and white haze – Emerging postharvest diseases of apple

With the introduction of new apple varieties, emerging diseases have been recorded including dry lenticel rot and white haze. Ramularia mali has been identified as the causal agent of dry lenticel rot, whereas species of GolubeviaTilletiopsis and Entyloma have been associated to white haze, but the epidemiology of these pathogens remains unclear. In the present study, we measured fruit disease incidence and quality parameters, and we used metabarcoding to characterize both epiphytic and endophytic microbial communities of apple fruit of two commercial cultivars, ‘Opal’ and ‘Ambrosia’, across six time points from early fruit development up to the end of shelf life. R. mali first develops in both cultivars as an endophyte at BBCH (Biologische Bundesanstalt, Bundessortenamt and CHemical industry) phenological phase 73 (10–11% relative abundance), BBCH 77 (26–33% relative abundance) and BBCH 81 (1–7% relative abundance), then it appears as an epiphyte from BBCH 87 onward (1–2% relative abundance), when symptoms start to be visible. This was confirmed in endophytic samples through qPCR specific for R. mali. Among the genera associated to white haze, Golubevia was the most abundant epiphyte (2–4%) from BBCH 81 to the end of shelf life. Alpha and beta diversity analyses unveiled the presence of significant difference both in richness and composition among different tissue, time points and cultivars. In conclusion, the study helps to explain the epidemiology of white haze and dry lenticel rot, and to design a targeted crop protection strategy, reinforcing the hypothesis that fruit metabarcoding could be a valuable tool for assessment and prediction of postharvest diseases, before symptoms occurrence in fruit.

Scientific Publication
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