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Gulzar A. Rather
Romica Verma
Bhavana Sharma
Arti Sharma
Anil Kumar

Usage of herbal medicines is extensive and increasing continuously. Their harvesting from wild habitats instigates the loss of genetic diversity. Cultivation at domestic level is a feasible alternative and provides an opportunity to overcome the inherent problems in herbal extracts: enhancing genetic and phenotypic variabilities, misidentification, extract variability and instability, toxic components, and impurities. Plant tissue cultures were performed for the preservation of medicinal wild resources as well for efficient production of pharmaceutically important compounds. In vitro organ culture can be used as an alternative strategy for the conservation of endangered genotypes, which can be achieved using different growth mediums and the sterile in vitro plants can be preserved via cryopreservation. Tissue culture helps to keep the genetic background of plants and to avoid the loss of the conserved heritage due to environmental changes, and stresses, either biotic or abiotic. Many seedless plants or plants having recalcitrant seeds cannot be stored in the long term. Such species can be conserved via in vitro techniques for their gene bank maintenance. Thus using tissue culture is crucial for the conservation of endangered medicinal plants and as a viable source material for biotechnological research activities.

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Chapter 17 - Tissue culture: A perpetual source for the conservation of medicinally important endangered plant species

Gulzar A. Rather
Romica Verma
Bhavana Sharma
Arti Sharma
Anil Kumar

Chapter 17 - Tissue culture: A perpetual source for the conservation of medicinally important endangered plant species

Usage of herbal medicines is extensive and increasing continuously. Their harvesting from wild habitats instigates the loss of genetic diversity. Cultivation at domestic level is a feasible alternative and provides an opportunity to overcome the inherent problems in herbal extracts: enhancing genetic and phenotypic variabilities, misidentification, extract variability and instability, toxic components, and impurities. Plant tissue cultures were performed for the preservation of medicinal wild resources as well for efficient production of pharmaceutically important compounds. In vitro organ culture can be used as an alternative strategy for the conservation of endangered genotypes, which can be achieved using different growth mediums and the sterile in vitro plants can be preserved via cryopreservation. Tissue culture helps to keep the genetic background of plants and to avoid the loss of the conserved heritage due to environmental changes, and stresses, either biotic or abiotic. Many seedless plants or plants having recalcitrant seeds cannot be stored in the long term. Such species can be conserved via in vitro techniques for their gene bank maintenance. Thus using tissue culture is crucial for the conservation of endangered medicinal plants and as a viable source material for biotechnological research activities.

Scientific Publication
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