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פותח על ידי קלירמאש פתרונות בע"מ -
Identification of WRKY transcription factors involved in regulating the biosynthesis of the anti-cancer drug camptothecin in BrowZine Ophiorrhiza pumila
Year:
2022
Source of publication :
Horticulture Research
Authors :
מעוז, איתי
;
.
Volume :
Co-Authors:

Can Wang 
Xiaolong Hao
Yao Wang
Itay Maoz
Wei Zhou
Zhigang Zhou
Guoyin Kai 

Facilitators :
From page:
0
To page:
0
(
Total pages:
1
)
Abstract:

Camptothecin is a chemotherapeutic drug widely used to treat various cancers. Ophiorrhiza pumila is an ideal plant model for the study of camptothecin production, with various advantages for studying camptothecin biosynthesis and regulation. The DNA-binding WRKY transcription factors have a key regulatory role in secondary metabolite biosynthesis in plants. However, little is currently known about their involvement in camptothecin biosynthesis in O. pumila. We identified 46 OpWRKY genes unevenly distributed on the 11 chromosomes of O. pumila. Phylogenetic and multiple sequence alignment analyses divided the OpWRKY proteins into three subfamilies. Based on spatial expression and co-expression, we targeted the candidate gene OpWRKY6. Overexpression of OpWRKY6 significantly reduced the accumulation of camptothecin compared with the control. Conversely, camptothecin accumulation increased in OpWRKY6 knockout lines. Further biochemical assays showed that OpWRKY6 negatively regulates camptothecin biosynthesis from both the iridoid and shikimate pathways by directly downregulating the gene expression of OpGESOp10HGOOp7DLH, and OpTDC. Our data provide direct evidence for the involvement of WRKYs in the regulation of camptothecin biosynthesis and offer valuable information for enriching the production of camptothecin in plant systems.

Note:
Related Files :
Ophiorrhiza pumila
WRKY transcription factor
עוד תגיות
תוכן קשור
More details
DOI :
10.1093/hr/uhac099
Article number:
0
Affiliations:
Database:
PubMed
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
63312
Last updated date:
24/01/2023 17:13
Creation date:
24/01/2023 17:13
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Scientific Publication
Identification of WRKY transcription factors involved in regulating the biosynthesis of the anti-cancer drug camptothecin in BrowZine Ophiorrhiza pumila

Can Wang 
Xiaolong Hao
Yao Wang
Itay Maoz
Wei Zhou
Zhigang Zhou
Guoyin Kai 

Identification of WRKY transcription factors involved in regulating the biosynthesis of the anti-cancer drug camptothecin in BrowZine Ophiorrhiza pumila

Camptothecin is a chemotherapeutic drug widely used to treat various cancers. Ophiorrhiza pumila is an ideal plant model for the study of camptothecin production, with various advantages for studying camptothecin biosynthesis and regulation. The DNA-binding WRKY transcription factors have a key regulatory role in secondary metabolite biosynthesis in plants. However, little is currently known about their involvement in camptothecin biosynthesis in O. pumila. We identified 46 OpWRKY genes unevenly distributed on the 11 chromosomes of O. pumila. Phylogenetic and multiple sequence alignment analyses divided the OpWRKY proteins into three subfamilies. Based on spatial expression and co-expression, we targeted the candidate gene OpWRKY6. Overexpression of OpWRKY6 significantly reduced the accumulation of camptothecin compared with the control. Conversely, camptothecin accumulation increased in OpWRKY6 knockout lines. Further biochemical assays showed that OpWRKY6 negatively regulates camptothecin biosynthesis from both the iridoid and shikimate pathways by directly downregulating the gene expression of OpGESOp10HGOOp7DLH, and OpTDC. Our data provide direct evidence for the involvement of WRKYs in the regulation of camptothecin biosynthesis and offer valuable information for enriching the production of camptothecin in plant systems.

Scientific Publication
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