חיפוש מתקדם
3 biotech
  • Kun Meng, 
  • Michal Eldar-Liebreich, 
  • Bhagwat Nawade, 
  • Mosaab Yahyaa, 
  • Liora Shaltiel-Harpaz, 
  • Moshe Coll, 
  • Asaf Sadeh 
    Mwafaq Ibdah 

Plant apocarotenoids have been shown to have a diverse biological role in herbivore–plant interactions. Despite their importance, little is known about herbivores’ effect on apocarotenoid emissions in Lactuca sativa. In this study, we examined changes in apocarotenoid emissions in lettuce leaves after infestation by two insects, viz., Spodoptera littoralis larvae and Myzus persicae aphids. We found that β-ionone and β-cyclocitral showed higher concentrations than the other apocarotenoids, with a significant increase as per the intensity of infestation of both herbivore species. Furthermore, we performed functional characterization of Lactuca sativa carotenoid cleavage dioxygenase 1 (LsCCD1) genes. Three LsCCD1 genes were overexpressed in E. coli strains, and recombinant proteins were assayed for cleavage activity on an array of carotenoid substrates. The LsCCD1 protein cleaved β-carotene at the 9,10 (9′,10′) positions producing β-ionone. The transcript analysis of LsCCD1 genes revealed differential expression patterns under varying levels of herbivores’ infestation, but the results were inconsistent with the pattern of β-ionone concentrations. Our results suggest that LsCCD1 is involved in the production of β-ionone, but other regulatory factors might be involved in its induction in response to herbivory. These results provide new insights into apocarotenoid production in response to insect herbivory in lettuce.

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תנאי שימוש
Analysis of apocarotenoid volatiles from lettuce (Lactuca sativa) induced by insect herbivores and characterization of carotenoid cleavage dioxygenase gene
  • Kun Meng, 
  • Michal Eldar-Liebreich, 
  • Bhagwat Nawade, 
  • Mosaab Yahyaa, 
  • Liora Shaltiel-Harpaz, 
  • Moshe Coll, 
  • Asaf Sadeh 
    Mwafaq Ibdah 
Analysis of apocarotenoid volatiles from lettuce (Lactuca sativa) induced by insect herbivores and characterization of carotenoid cleavage dioxygenase gene

Plant apocarotenoids have been shown to have a diverse biological role in herbivore–plant interactions. Despite their importance, little is known about herbivores’ effect on apocarotenoid emissions in Lactuca sativa. In this study, we examined changes in apocarotenoid emissions in lettuce leaves after infestation by two insects, viz., Spodoptera littoralis larvae and Myzus persicae aphids. We found that β-ionone and β-cyclocitral showed higher concentrations than the other apocarotenoids, with a significant increase as per the intensity of infestation of both herbivore species. Furthermore, we performed functional characterization of Lactuca sativa carotenoid cleavage dioxygenase 1 (LsCCD1) genes. Three LsCCD1 genes were overexpressed in E. coli strains, and recombinant proteins were assayed for cleavage activity on an array of carotenoid substrates. The LsCCD1 protein cleaved β-carotene at the 9,10 (9′,10′) positions producing β-ionone. The transcript analysis of LsCCD1 genes revealed differential expression patterns under varying levels of herbivores’ infestation, but the results were inconsistent with the pattern of β-ionone concentrations. Our results suggest that LsCCD1 is involved in the production of β-ionone, but other regulatory factors might be involved in its induction in response to herbivory. These results provide new insights into apocarotenoid production in response to insect herbivory in lettuce.

Scientific Publication
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