Satish Kumar Rajasekharan
Moshe Shemesh

Food and its contact surfaces as well as processing units are common sites of contamination by food spoilage microorganisms. Most resilient microorganisms to conventional treatment procedures are pathogenic species that often form robust biofilms and threaten food safety. Therefore, alternative antimicrobial therapies must be explored to successfully control potential outbreaks caused by biofilm-forming foodborne pathogens Application of probiotics to fight pathogenic biofilms is a modest procedure that appears to be useful in improving food safety and functionality. For instance, probiotic Bacilli and their by-products (herein postbiotics) have been demonstrated for its antagonistic role against foodborne pathogens. In addition, these bacteria also possess remarkable ability to colonize food matrices. Food matrices or prebiotics that aid probiotic colonization are one well-known and promising technique of delivering probiotics. Prebiotics are nondigestible fiber components of food that travel almost intact through the GI tract and serve as nutritional feed for health-promoting gut bacteria. When administered independently, both prebiotics and probiotics have health benefits. Prebiotics are, interestingly, the sites of probiotic colonization, and the two combined create a probiotic-prebiotic complex, also known as synbiotics. Overall, the chapter explores the use of Bacilli to control foodborne pathogens as well as the feasibility of using legume-based prebiotic dietary fibers as a natural scaffold for growing Bacilli to develop synbiotic food for nutritional benefits and to fight biofilm-forming pathogens.