חיפוש מתקדם
Postharvest Biology and Technology

Jing Yuan
Yongxiang Liu
Yongcai Li
Yuanyuan Zong
Yang Bi
Dov B. Prusky 

Sln, a conserved sensor kinase, plays a distinct function in different fungi. The gene AaSln1 in Alternaria alternata was successfully cloned and characterized. Sequence analysis revealed that AaSln1 consists of a transmembrane helix region, two characteristic low complexity regions, and a HisKA, HATPase_c and sesponse reg domain, respectively. Through gene knockout strategy, the results showed that AaSln1 significantly regulated infection structural differentiation, pathogenicity, stress response and melanin synthesis and secretion, while had no effect on growth, sporulation and biomass accumulation of A. alternata. The AaSln1 regulates pathways in A. alternata including ABC transportersstarch and sucrose metabolism according to the transcriptomic analysis. RNA-Seq results revealed that the gene expression levels of the downstream regulators of Hog1-MAPK pathway such as Mss4Ste12Fsk2 and Mip1 were significantly reduced in the ΔAaSln1 mutant. These findings demonstrated that AaSln1 has an essential role in the pathogenicity and regulates downstream biological processes of A. alternata by activating the MAPK pathway.

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תנאי שימוש
The sensor protein AaSln1 is involved in differentiation of infection structures, osmotic stress tolerance and virulence in Alternaria alternata
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Jing Yuan
Yongxiang Liu
Yongcai Li
Yuanyuan Zong
Yang Bi
Dov B. Prusky 

The sensor protein AaSln1 is involved in differentiation of infection structures, osmotic stress tolerance and virulence in Alternaria alternata

Sln, a conserved sensor kinase, plays a distinct function in different fungi. The gene AaSln1 in Alternaria alternata was successfully cloned and characterized. Sequence analysis revealed that AaSln1 consists of a transmembrane helix region, two characteristic low complexity regions, and a HisKA, HATPase_c and sesponse reg domain, respectively. Through gene knockout strategy, the results showed that AaSln1 significantly regulated infection structural differentiation, pathogenicity, stress response and melanin synthesis and secretion, while had no effect on growth, sporulation and biomass accumulation of A. alternata. The AaSln1 regulates pathways in A. alternata including ABC transportersstarch and sucrose metabolism according to the transcriptomic analysis. RNA-Seq results revealed that the gene expression levels of the downstream regulators of Hog1-MAPK pathway such as Mss4Ste12Fsk2 and Mip1 were significantly reduced in the ΔAaSln1 mutant. These findings demonstrated that AaSln1 has an essential role in the pathogenicity and regulates downstream biological processes of A. alternata by activating the MAPK pathway.

Scientific Publication
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