Co-Authors:
Cohen, J., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Sikron, N., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Shuval, S., Danziger Flower Farm, Mishmar Hashiva 50277, Israel
Gera, A., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Abstract:
In this study, 18 Petunia xhybrida Hort. Volm.-Andr. cultivars were mechanically inoculated with the tobamoviruses tobacco mosaic (TMV) or tomato mosaic virus (ToMV) (20 μg·L-1 in 0.05 M sodium phosphate buffer). One and 2 weeks post-inoculation (PI), inoculated and noninoculated upper leaves were harvested and assayed for TMV infection using enzyme-linked immunosorbent assay (ELISA). Local lesions developed on inoculated leaves of 16 cultivars 3-5 days PI. A total of 11 and 16 of the cultivars developed systemic symptoms characteristic of tobamovirus infection 2 weeks after inoculation with TMV and ToMV, respectively. All cultivars were positive in ELISA tests. Large amounts of virus were recovered from the upper, noninoculated leaves of all cultivars, including symptomless plants. Up to 95% infection by TMV occurred when a sterilized knife was passed through an infected shoot of petunia prior to its being used to remove cuttings from healthy petunia plants. Heat sterilization of knives and/or treatment with 2.8 g·L-1 sodium troclosene was very effective in controlling TMV transmission.
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