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R-Ras3/(M-Ras) is involved in thermal adaptation in the critical period of thermal control establishment
Year:
2006
Source of publication :
Journal of Neurobiology
Authors :
Labunskay, Galya
;
.
Meiri, Noam
;
.
Volume :
66
Co-Authors:
Labunskay, G., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Meiri, N., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
56
To page:
70
(
Total pages:
15
)
Abstract:
Neuroanatomically, the body temperature is balanced by the preoptic anterior hypothalamus (PO/AH) and controlled by thermosensitive neurons. Hot or cold exposure during the critical period of temperature control development causes a plastic change in the ratio between hot- and cold-sensitive cells and can modulate temperature tolerance. In this project, mRNA fingerprinting was used to identify the proteins involved in thermal adaptation in 3-day-old chicks. Fifteen genes were induced, among which were NADH dehydrogenase, protocadherin, anolase α, 14-3-3ε, and R-Ras3. The role of each of these genes is potentially interesting and requires detailed evaluation, but since the present working hypothesis assumed neuronal remodeling, we concentrated on the role of R-Ras3/(M-Ras), which is uniquely expressed in the brain and whose physiological role has not been described. In the present study, R-Ras3 expression during thermal conditioning was investigated by several molecular techniques and its mRNA was found to be induced in the PO/AH with a tenfold peak after 12 h of heat conditioning and a fourfold increase after 6 h of cold conditioning. To improve our understanding of thermal adaptation-related signal transduction, we screened for changes in the expression of transcription factors that were implicated with the Ras gene family, and found that both jun mRNA expression and Jun phosphorylation were induced after 30 min of temperature conditioning. Taken together, the present findings correlate the R-Ras3-jun pathway with thermal-control establishment. © 2005 Wiley Periodicals, Inc.
Note:
Related Files :
animal experiment
Animals
chicks
gene expression
heat tolerance
Male
reduced nicotinamide adenine dinucleotide dehydrogenase
Show More
Related Content
More details
DOI :
10.1002/neu.20191
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
18447
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:21
Scientific Publication
R-Ras3/(M-Ras) is involved in thermal adaptation in the critical period of thermal control establishment
66
Labunskay, G., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Meiri, N., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
R-Ras3/(M-Ras) is involved in thermal adaptation in the critical period of thermal control establishment
Neuroanatomically, the body temperature is balanced by the preoptic anterior hypothalamus (PO/AH) and controlled by thermosensitive neurons. Hot or cold exposure during the critical period of temperature control development causes a plastic change in the ratio between hot- and cold-sensitive cells and can modulate temperature tolerance. In this project, mRNA fingerprinting was used to identify the proteins involved in thermal adaptation in 3-day-old chicks. Fifteen genes were induced, among which were NADH dehydrogenase, protocadherin, anolase α, 14-3-3ε, and R-Ras3. The role of each of these genes is potentially interesting and requires detailed evaluation, but since the present working hypothesis assumed neuronal remodeling, we concentrated on the role of R-Ras3/(M-Ras), which is uniquely expressed in the brain and whose physiological role has not been described. In the present study, R-Ras3 expression during thermal conditioning was investigated by several molecular techniques and its mRNA was found to be induced in the PO/AH with a tenfold peak after 12 h of heat conditioning and a fourfold increase after 6 h of cold conditioning. To improve our understanding of thermal adaptation-related signal transduction, we screened for changes in the expression of transcription factors that were implicated with the Ras gene family, and found that both jun mRNA expression and Jun phosphorylation were induced after 30 min of temperature conditioning. Taken together, the present findings correlate the R-Ras3-jun pathway with thermal-control establishment. © 2005 Wiley Periodicals, Inc.
Scientific Publication
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