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Inoculation, isolation and identification of Tuber melanosporum from old and new oak hosts in Israel
Year:
2000
Source of publication :
Mycological Research
Authors :
Freeman, Stanley
;
.
Maymon, Marcel
;
.
Salomon, Elisha
;
.
Shabi, Ezra
;
.
Shmulevich, Yehuda
;
.
Volume :
104
Co-Authors:
Pinkas, Y., Department of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel
Maimon, M., Department of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel
Shabi, E., Department of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel
Elisha, S., Department of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel
Shmulewich, Y., Department of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel
Freeman, S., Department of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
472
To page:
477
(
Total pages:
6
)
Abstract:
Five local oak species, Quercus boissieri, Q. calliprinus, Q. cerris, Q. ithaburensis and Q. libani were identified as hosts of Tuber melanosporum, the black truffle of Perigord, following inoculation of roots Q. cerris and Q. libani developed abundant mycorrhizal roots, similar to the amount found in roots of Q. pubescens, the traditional host of this mycorrhizal fungus. Roots of Q. ilex, Q. hartwissiana and Q. pedunculiflora, introduced species in Israel, were also heavily colonized by the mycorrhizal fungus. Mycorrhized hazel (Corylus avellana) roots, were also obtained by the same inoculation procedure. Recovery of T. melanosporum from roots of inoculated oak was improved when chloramphenicol was added to the isolation medium. Recovery varied between 14 and 54% for C. avellana and Q. calliprinus, respectively. Recovery was highest for the two local species, Q. calliprinus (54%) and Q. boissieri (50%), followed by Q. pubescens (44%) and Q ilex (41%). Identification of the isolated fungi was conducted using arbitrarily-primed PCR. Identical band patterns were observed among AP-PCR-amplified DNA extracted from an authentic culture of the fungus, ascocarps (truffles) and cultures isolated from roots. In addition, AP-PCR was reliable for differentiating between representative isolates of T. melanosporum, T. magnatum, T. borchii, T. maculatum, T. dryophilum, T. macrosporum and T. uncinatum.
Note:
Related Files :
fungus detection
Inoculation
Israel
Mycorrhiza
Quercus pedunculiflora
Quercus pubescens
Tuber uncinatum
Show More
Related Content
More details
DOI :
10.1017/S0953756299001458
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
18480
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:22
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Scientific Publication
Inoculation, isolation and identification of Tuber melanosporum from old and new oak hosts in Israel
104
Pinkas, Y., Department of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel
Maimon, M., Department of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel
Shabi, E., Department of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel
Elisha, S., Department of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel
Shmulewich, Y., Department of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel
Freeman, S., Department of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel
Inoculation, isolation and identification of Tuber melanosporum from old and new oak hosts in Israel
Five local oak species, Quercus boissieri, Q. calliprinus, Q. cerris, Q. ithaburensis and Q. libani were identified as hosts of Tuber melanosporum, the black truffle of Perigord, following inoculation of roots Q. cerris and Q. libani developed abundant mycorrhizal roots, similar to the amount found in roots of Q. pubescens, the traditional host of this mycorrhizal fungus. Roots of Q. ilex, Q. hartwissiana and Q. pedunculiflora, introduced species in Israel, were also heavily colonized by the mycorrhizal fungus. Mycorrhized hazel (Corylus avellana) roots, were also obtained by the same inoculation procedure. Recovery of T. melanosporum from roots of inoculated oak was improved when chloramphenicol was added to the isolation medium. Recovery varied between 14 and 54% for C. avellana and Q. calliprinus, respectively. Recovery was highest for the two local species, Q. calliprinus (54%) and Q. boissieri (50%), followed by Q. pubescens (44%) and Q ilex (41%). Identification of the isolated fungi was conducted using arbitrarily-primed PCR. Identical band patterns were observed among AP-PCR-amplified DNA extracted from an authentic culture of the fungus, ascocarps (truffles) and cultures isolated from roots. In addition, AP-PCR was reliable for differentiating between representative isolates of T. melanosporum, T. magnatum, T. borchii, T. maculatum, T. dryophilum, T. macrosporum and T. uncinatum.
Scientific Publication
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