Novel technique for quantifying adhesion of metarhizium anisopliae conidia, to the tick cuticle

Hello

English

Quick User Guide

Search...

Volcani treasures

About

Novel technique for quantifying adhesion of metarhizium anisopliae conidia, to the tick cuticle

Year: 

2010

Source of publication :

Applied and Environmental Microbiology

Authors :

Gindin, Galina

;

Glazer, Itamar

;

Ment, Dana

;

Samish, Michael

;

Soroker, Victoria

.

Volume :

Co-Authors: 

Ment, D., ARO, Volcani Center, P.O.B. 6, Bet-Dagan 50250, Israel
Gindin, G., ARO, Volcani Center, P.O.B. 6, Bet-Dagan 50250, Israel
Rot, A., ARO, Kimron Veterinary Institute, P.O.B. 12, Bet-Dagan 50250, Israel
Soroker, V., ARO, Volcani Center, P.O.B. 6, Bet-Dagan 50250, Israel
Glazer, I., ARO, Volcani Center, P.O.B. 6, Bet-Dagan 50250, Israel
Barel, S., ARO, Kimron Veterinary Institute, P.O.B. 12, Bet-Dagan 50250, Israel
Samish, M., ARO, Kimron Veterinary Institute, P.O.B. 12, Bet-Dagan 50250, Israel

From page: 

3521

To page: 

3528

(

Total pages: 

8

)

Link :

Novel technique for quantifying adhesion of metarhizium anisopliae conidia, to the tick cuticle

Abstract: 

The present study describes an accurate quantitative method for quantifying the adherence of conidia to the arthropod cuticle and the dynamics of conidial germination on the host. The method was developed using conidia of Metarhizium anisopliae var. anisopliae (Metschn.) Sorokin (Hypocreales: Clavicipitaceae) and engorged Rhipicephalus annulatus (Say) (Arachnida: Ixodidae) females and was also verified for M. anisopliae var. acridum Driver et Milner (Hypocreales: Clavicipitaceae) and Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae) larvae. This novel method is based on using an organic solvent (dichloromethane [DCM]) to remove the adhered conidia from the tick cuticle, suspending the conidia in a detergent solution, and then counting them using a hemocytometer. To confirm the efficacy of the method, scanning electron microscopy (SEM) was used to observe the conidial adherence to and removal from the tick cuticle. As the concentration of conidia in the suspension increased, there were correlating increases in both the number of conidia adhering to engorged female R. annulatus and tick mortality. However, no correlation was observed between a tick's susceptibility to fungal infection and the amount of adhered conidia. These findings support the commonly accepted understanding of the nature of the adhesion process. The mechanism enabling the removal of the adhered conidia from the host cuticle is discussed. Copyright © 2010, American society tor Microbiology. All Rights Reserved.