Abstract:
Sitophilus oryzae adults were exposed to 40% carbon dioxide (CO2) in air for seven successive generations and to 75% CO2 in air for ten successive generations at 26°C and 100% relative humidity (r.h.). The tolerance factor (LT95 selected generation/LT95 non-selected generation) at the 7th generation of insects exposed to 40% CO2 was 2.15 and at the 10th generation exposed to 75% CO2, was 3.34. Reduction of the r.h. to 60% in the 75% CO2 atmosphere caused shortening of exposure times required to obtain the same mortality values, but the tolerance factors remained stable. Removal of selection pressure for five generations of the 40% CO2 and four generations of the 75% CO2 selected strains caused significant reduction in tolerance. Augmentation of oxygen concentration to 21% in atmospheres containing 40 and 75% CO2 did not markedly alter the tolerance factors from those obtained with atmospheres containing the same CO2 concentrations in air. © 1985.
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