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Theoretical and Applied Genetics
Ben-David, R., Department of Evolutionary and Environmental Biology, The Institute of Evolution, Faculty of Science and Science Education, University of Haifa, Mt. Carmel, 31905 Haifa, Israel
Xie, W., Department of Evolutionary and Environmental Biology, The Institute of Evolution, Faculty of Science and Science Education, University of Haifa, Mt. Carmel, 31905 Haifa, Israel
Peleg, Z., Department of Evolutionary and Environmental Biology, The Institute of Evolution, Faculty of Science and Science Education, University of Haifa, Mt. Carmel, 31905 Haifa, Israel, Department of Plant Sciences, University of California, Davis, CA 95616, United States
Saranga, Y., Institute of Plant Science and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, 76100 Rehovot, Israel
Dinoor, A., Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, 76100 Rehovot, Israel
Fahima, T., Department of Evolutionary and Environmental Biology, The Institute of Evolution, Faculty of Science and Science Education, University of Haifa, Mt. Carmel, 31905 Haifa, Israel
The gene-pool of wild emmer wheat, Triticum turgidum ssp. dicoccoides, harbors a rich allelic repertoire for disease resistance. In the current study, we made use of tetraploid wheat mapping populations derived from a cross between durum wheat (cv. Langdon) and wild emmer (accession G18-16) to identify and map a new powdery mildew resistance gene derived from wild emmer wheat. Initially, the two parental lines were screened with a collection of 42 isolates of Blumeria graminis f. sp. tritici (Bgt) from Israel and 5 isolates from Switzerland. While G18-16 was resistant to 34 isolates, Langdon was resistant only to 5 isolates and susceptible to 42 isolates. Isolate Bgt#15 was selected to differentiate between the disease reactions of the two genotypes. Segregation ratio of F2-3 and recombinant inbreed line (F7) populations to inoculation with isolate Bgt#15 indicated the role of a single dominant gene in conferring resistance to Bgt#15. This gene, temporarily designated PmG16, was located on the distal region of chromosome arm 7AL. Genetic map of PmG16 region was assembled with 32 simple sequence repeat (SSR), sequence tag site (STS), Diversity array technology (DArT) and cleaved amplified polymorphic sequence (CAPS) markers and assigned to the 7AL physical bin map (7AL-16). Using four DNA markers we established colinearity between the genomic region spanning the PmG16 locus within the distal region of chromosome arm 7AL and the genomic regions on rice chromosome 6 and Brachypodium Bd1. A comparative analysis was carried out between PmG16 and other known Pm genes located on chromosome arm 7AL. The identified PmG16 may facilitate the use of wild alleles for improvement of powdery mildew resistance in elite wheat cultivars via marker-assisted selection. © 2010 Springer-Verlag.
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Identification and mapping of PmG16, a powdery mildew resistance gene derived from wild emmer wheat
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Ben-David, R., Department of Evolutionary and Environmental Biology, The Institute of Evolution, Faculty of Science and Science Education, University of Haifa, Mt. Carmel, 31905 Haifa, Israel
Xie, W., Department of Evolutionary and Environmental Biology, The Institute of Evolution, Faculty of Science and Science Education, University of Haifa, Mt. Carmel, 31905 Haifa, Israel
Peleg, Z., Department of Evolutionary and Environmental Biology, The Institute of Evolution, Faculty of Science and Science Education, University of Haifa, Mt. Carmel, 31905 Haifa, Israel, Department of Plant Sciences, University of California, Davis, CA 95616, United States
Saranga, Y., Institute of Plant Science and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, 76100 Rehovot, Israel
Dinoor, A., Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, 76100 Rehovot, Israel
Fahima, T., Department of Evolutionary and Environmental Biology, The Institute of Evolution, Faculty of Science and Science Education, University of Haifa, Mt. Carmel, 31905 Haifa, Israel
Identification and mapping of PmG16, a powdery mildew resistance gene derived from wild emmer wheat
The gene-pool of wild emmer wheat, Triticum turgidum ssp. dicoccoides, harbors a rich allelic repertoire for disease resistance. In the current study, we made use of tetraploid wheat mapping populations derived from a cross between durum wheat (cv. Langdon) and wild emmer (accession G18-16) to identify and map a new powdery mildew resistance gene derived from wild emmer wheat. Initially, the two parental lines were screened with a collection of 42 isolates of Blumeria graminis f. sp. tritici (Bgt) from Israel and 5 isolates from Switzerland. While G18-16 was resistant to 34 isolates, Langdon was resistant only to 5 isolates and susceptible to 42 isolates. Isolate Bgt#15 was selected to differentiate between the disease reactions of the two genotypes. Segregation ratio of F2-3 and recombinant inbreed line (F7) populations to inoculation with isolate Bgt#15 indicated the role of a single dominant gene in conferring resistance to Bgt#15. This gene, temporarily designated PmG16, was located on the distal region of chromosome arm 7AL. Genetic map of PmG16 region was assembled with 32 simple sequence repeat (SSR), sequence tag site (STS), Diversity array technology (DArT) and cleaved amplified polymorphic sequence (CAPS) markers and assigned to the 7AL physical bin map (7AL-16). Using four DNA markers we established colinearity between the genomic region spanning the PmG16 locus within the distal region of chromosome arm 7AL and the genomic regions on rice chromosome 6 and Brachypodium Bd1. A comparative analysis was carried out between PmG16 and other known Pm genes located on chromosome arm 7AL. The identified PmG16 may facilitate the use of wild alleles for improvement of powdery mildew resistance in elite wheat cultivars via marker-assisted selection. © 2010 Springer-Verlag.
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