אסיף מאגר המחקר החקלאי

Putrescine and hydrogen peroxide improve the rooting of 'GF-677' rootstock in woody cuttings and tissue culture shoots

Year:

2006

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Volume :

713

Co-Authors:

Zilkah, S., Institute of Horticulture, Volcani Center, Israel
Zamiri, N., Institute of Horticulture, Volcani Center, Israel
Ziv, M., Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, Hebrew University of Jerusalem, Israel

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Abstract:

'GF-677', a rootstock hybrid of peach X almond, has an economic and horticultural importance in the plantation and especially in replantation of peach and other stone fruits. The rootstock cuttings are difficult-to-propagate, which constitutes a problem in supplying the rootstock demand. Putrescine and hydrogen peroxide were found to be involved in the rooting process. Therefore, their effects on rooting of 'GF-677' of in vivo and in vitro systems are being addressed. Putrescine and hydrogen peroxide doubled the percentage of the rooted 'GF-677' cuttings, relatively to IBA alone. Nevertheless, the maximal rooting percent did not exceed 27%. In the tissue culture system, in the absence of IBA in the medium, putrescine (1 mM) and hydrogen peroxide (dipping 1 M solution for 2 s) raised the explants rooting percentage from 0 to 42% and 35%, respectively. In the presence of IBA, both tested substances increased, not significantly, the rooting percentage, which was anyhow 70%. However, hydrogen peroxide increased the weight (190%), number (150%) and length (64%) of roots per explant. Putrescine increased significantly the weight (110%) and the number (106%) of roots per explant. It appears that the rooting of 'GF-677' rootstock could be significantly improved by the addition of putrescine and hydrogen peroxide to the rooting medium under in vivo and in vitro systems.

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