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Molecular characterisation of Onion yellow dwarf virus (OYDV) infecting garlic (Allium sativum L.) in Israel: Thermotherapy inhibits virus elimination by meristem tip culture
Year:
2001
Source of publication :
Annals of Applied Biology
Authors :
Gal-On, Amit
;
.
Koch-Dean, Margery
;
.
Salomon, Raffi
;
.
Volume :
138
Co-Authors:
Shiboleth, Y.M., Dept. of Virology, ARO, Volcani Center, Bet Dagan 50250, Israel
Gal-On, A., Dept. of Virology, ARO, Volcani Center, Bet Dagan 50250, Israel
Koch, M., Dept. of Vegetable Crops., ARO, Volcani Center, Bet Dagan 50250, Israel
Rabinowitch, H.D., Dept. of Field Crops, Vegetables and Genetics, Hebrew University of Jerusalem, Faculty of Agricultural, Food and Environmental Sciences, Rehovot 76100, Israel
Salomon, R., Dept. of Virology, ARO, Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
187
To page:
195
(
Total pages:
9
)
Abstract:
Garlic (cv. Shani) was tested using single step RT-PCR and digoxygenin (DIG) labelled dot-blot for a number of viruses. Following sequence analysis it was shown that at least three different polymorphs of the potyvirus Onion yellow dwarf virus (OYDV) infect the same plant simultaneously, together with the potyvirus Leek yellow stripe virus (LYSV), the carlavirus Garlic common latent virus (GCLV) and a multitude of allexiviruses [Shallot virus X (ShVX) related viruses]. Several garlic plants free of all the viruses tested were obtained through meristem-tip culture. Plants infected with single viruses or with different combinations of viruses were similarly obtained. Meristem-tip culture was confirmed as a satisfactory method of virus eradication, while thermotherapy treatment given to mother plantlets before meristem excision was found to specifically antagonise OYDV eradication. This work uses molecular methods for the first time to examine the effectiveness of meristem-tip culture for the eradication of multiple viruses from garlic.
Note:
Related Files :
Allium cepa
Allium sativum
digoxygenin
Israel
meristem
Onion yellow dwarf virus (OYDV)
Shallot virus X (ShVX)
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More details
DOI :
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
18849
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:24
Scientific Publication
Molecular characterisation of Onion yellow dwarf virus (OYDV) infecting garlic (Allium sativum L.) in Israel: Thermotherapy inhibits virus elimination by meristem tip culture
138
Shiboleth, Y.M., Dept. of Virology, ARO, Volcani Center, Bet Dagan 50250, Israel
Gal-On, A., Dept. of Virology, ARO, Volcani Center, Bet Dagan 50250, Israel
Koch, M., Dept. of Vegetable Crops., ARO, Volcani Center, Bet Dagan 50250, Israel
Rabinowitch, H.D., Dept. of Field Crops, Vegetables and Genetics, Hebrew University of Jerusalem, Faculty of Agricultural, Food and Environmental Sciences, Rehovot 76100, Israel
Salomon, R., Dept. of Virology, ARO, Volcani Center, Bet Dagan 50250, Israel
Molecular characterisation of Onion yellow dwarf virus (OYDV) infecting garlic (Allium sativum L.) in Israel: Thermotherapy inhibits virus elimination by meristem tip culture
Garlic (cv. Shani) was tested using single step RT-PCR and digoxygenin (DIG) labelled dot-blot for a number of viruses. Following sequence analysis it was shown that at least three different polymorphs of the potyvirus Onion yellow dwarf virus (OYDV) infect the same plant simultaneously, together with the potyvirus Leek yellow stripe virus (LYSV), the carlavirus Garlic common latent virus (GCLV) and a multitude of allexiviruses [Shallot virus X (ShVX) related viruses]. Several garlic plants free of all the viruses tested were obtained through meristem-tip culture. Plants infected with single viruses or with different combinations of viruses were similarly obtained. Meristem-tip culture was confirmed as a satisfactory method of virus eradication, while thermotherapy treatment given to mother plantlets before meristem excision was found to specifically antagonise OYDV eradication. This work uses molecular methods for the first time to examine the effectiveness of meristem-tip culture for the eradication of multiple viruses from garlic.
Scientific Publication
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