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Biolistic inoculation of plants with Tomato yellow leaf curl virus DNA
Year:
2007
Source of publication :
Journal of Virological Methods
Authors :
Cohen, Lydia
;
.
Gaba, Victor
;
.
Lapidot, Moshe
;
.
Segev, Limor
;
.
Weil, Galit
;
.
Volume :
144
Co-Authors:
Lapidot, M., Department of Vegetable Research, Institute of Plant Sciences, ARO Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Weil, G., Department of Vegetable Research, Institute of Plant Sciences, ARO Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Cohen, L., Department of Vegetable Research, Institute of Plant Sciences, ARO Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Segev, L., Department of Vegetable Research, Institute of Plant Sciences, ARO Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Gaba, V., Department of Plant Pathology and Weed Science, Institute of Plant Protection, ARO Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Facilitators :
From page:
143
To page:
148
(
Total pages:
6
)
Abstract:
Tomato yellow leaf curl virus (TYLCV) full-length DNA was amplified by PCR and cloned into a bacterial plasmid. The cloned TYLCV DNA was excised from the plasmid, ligated and the resulting monomeric circular double-stranded TYLCV DNA was used to inoculate tomato (Solanum lycopersicom) and datura (Datura stramonium) plants by particle bombardment. The bombarded plants produced typical disease symptoms, similar to those produced following whitefly-mediated inoculation, albeit 5-7 days later than whitefly-inoculated plants. The success rate of inoculating tomato plants by particle bombardment averaged 37%, whereas with datura plants, it averaged 85%. With whitefly-mediated inoculation of TYLCV, the success rate of inoculation was also higher in datura plants than in tomato plants. Bombardment of datura plants with a linear form of TYLCV DNA also resulted in viral infection, with an inoculation success rate similar to that with the closed-circular TYLCV DNA. Bombarding datura plants with the bacterial plasmid containing the cloned TYLCV DNA did not result in viral infection, but bombardment with a bacterial plasmid containing a cloned dimer of TYLCV DNA yielded an infection rate of 50-100%. This is the first report of TYLCV inoculation of plants using particle bombardment of a cloned monomeric linear or closed-circular form of TYLCV double-stranded DNA. © 2007 Elsevier B.V. All rights reserved.
Note:
Related Files :
Animals
biolistic transformation
cloning
Datura
Datura stramonium
Plant Diseases
Plasmid
Solanum
Tomato yellow leaf curl virus
Show More
Related Content
More details
DOI :
10.1016/j.jviromet.2007.04.011
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
18875
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:24
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Scientific Publication
Biolistic inoculation of plants with Tomato yellow leaf curl virus DNA
144
Lapidot, M., Department of Vegetable Research, Institute of Plant Sciences, ARO Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Weil, G., Department of Vegetable Research, Institute of Plant Sciences, ARO Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Cohen, L., Department of Vegetable Research, Institute of Plant Sciences, ARO Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Segev, L., Department of Vegetable Research, Institute of Plant Sciences, ARO Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Gaba, V., Department of Plant Pathology and Weed Science, Institute of Plant Protection, ARO Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Biolistic inoculation of plants with Tomato yellow leaf curl virus DNA
Tomato yellow leaf curl virus (TYLCV) full-length DNA was amplified by PCR and cloned into a bacterial plasmid. The cloned TYLCV DNA was excised from the plasmid, ligated and the resulting monomeric circular double-stranded TYLCV DNA was used to inoculate tomato (Solanum lycopersicom) and datura (Datura stramonium) plants by particle bombardment. The bombarded plants produced typical disease symptoms, similar to those produced following whitefly-mediated inoculation, albeit 5-7 days later than whitefly-inoculated plants. The success rate of inoculating tomato plants by particle bombardment averaged 37%, whereas with datura plants, it averaged 85%. With whitefly-mediated inoculation of TYLCV, the success rate of inoculation was also higher in datura plants than in tomato plants. Bombardment of datura plants with a linear form of TYLCV DNA also resulted in viral infection, with an inoculation success rate similar to that with the closed-circular TYLCV DNA. Bombarding datura plants with the bacterial plasmid containing the cloned TYLCV DNA did not result in viral infection, but bombardment with a bacterial plasmid containing a cloned dimer of TYLCV DNA yielded an infection rate of 50-100%. This is the first report of TYLCV inoculation of plants using particle bombardment of a cloned monomeric linear or closed-circular form of TYLCV double-stranded DNA. © 2007 Elsevier B.V. All rights reserved.
Scientific Publication
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