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The fate of radiolabeled steroids in ovaries and eggs of the tobacco hornworm, Manduca sexta
Year:
1986
Source of publication :
Lipids
Authors :
Svoboda, James
;
.
Volume :
21
Co-Authors:
Thompson, M.J., Insect Physiology Laboratory, Agricultural Research Service, USDA, Building 467, BARC-East, Beltsville, 20705, Maryland, United States
Svoboda, J.A., Insect Physiology Laboratory, Agricultural Research Service, USDA, Building 467, BARC-East, Beltsville, 20705, Maryland, United States
Feldlaufer, M.F., Insect Physiology Laboratory, Agricultural Research Service, USDA, Building 467, BARC-East, Beltsville, 20705, Maryland, United States
Lozano, R., Insect Physiology Laboratory, Agricultural Research Service, USDA, Building 467, BARC-East, Beltsville, 20705, Maryland, United States
Facilitators :
From page:
76
To page:
81
(
Total pages:
6
)
Abstract:
To determine the precursors and the fate of 26-hydroxyecdysone in eggs, the fate of labeled putative ecdysteroid precursors was examined in the tobacco hornworm, Manduca sexta. Following injection of [14C]cholesterol, 22,25[14C]dideoxyecdysome or [3H]ecdysone into female pupae (day 16), only [14C]cholesterol was incorporated and metabolized. It was converted to labeled nonecdysteroid and ecdysteroid conjugates, of which the latter in ovaries and 48- to 64-hr-old eggs is mainly 26-hydroxyecdysone 26-phosphate (>85% in ovaries). Quantitation of the ecdysteroid conjugate by reversed-phase high performance liquid chromatography (RP-HPLC) showed that the levels of 26-hydroxyecdysone 26-phosphate were 31 μg/g of ovaries from 4-day-old females and 25 μg/g and 17 μg/g of 48- to 64-hr-old and 72- to 88-hr-old eggs, respectively. The RP-HPLC of the conjugate fraction of 48- to 64-hr-old eggs showed an additional peak of radioactive material eluting about three min before the 26-hydroxyecdysone 26-phosphate. The quantity of this material increased in the 72-to 88-hr-old eggs, though it was not detected in the analyses of the conjugate fraction from ovaries. Additional peaks of radioactive material eluting before the 26-hydroxyecdysone 26-phosphate peak were observed in the chromatogram of the conjugates of 72- to 88-hr-old eggs. These radioactive materials need to be identified to determine the ultimate fate of ecdysteroids in the developing embryos of the tobacco hornworm. No radioactive free ecdysteroids were detected in either egg age group. © 1986 American Oil Chemists' Society.
Note:
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DOI :
10.1007/BF02534306
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
18879
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:24
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Scientific Publication
The fate of radiolabeled steroids in ovaries and eggs of the tobacco hornworm, Manduca sexta
21
Thompson, M.J., Insect Physiology Laboratory, Agricultural Research Service, USDA, Building 467, BARC-East, Beltsville, 20705, Maryland, United States
Svoboda, J.A., Insect Physiology Laboratory, Agricultural Research Service, USDA, Building 467, BARC-East, Beltsville, 20705, Maryland, United States
Feldlaufer, M.F., Insect Physiology Laboratory, Agricultural Research Service, USDA, Building 467, BARC-East, Beltsville, 20705, Maryland, United States
Lozano, R., Insect Physiology Laboratory, Agricultural Research Service, USDA, Building 467, BARC-East, Beltsville, 20705, Maryland, United States
The fate of radiolabeled steroids in ovaries and eggs of the tobacco hornworm, Manduca sexta
To determine the precursors and the fate of 26-hydroxyecdysone in eggs, the fate of labeled putative ecdysteroid precursors was examined in the tobacco hornworm, Manduca sexta. Following injection of [14C]cholesterol, 22,25[14C]dideoxyecdysome or [3H]ecdysone into female pupae (day 16), only [14C]cholesterol was incorporated and metabolized. It was converted to labeled nonecdysteroid and ecdysteroid conjugates, of which the latter in ovaries and 48- to 64-hr-old eggs is mainly 26-hydroxyecdysone 26-phosphate (>85% in ovaries). Quantitation of the ecdysteroid conjugate by reversed-phase high performance liquid chromatography (RP-HPLC) showed that the levels of 26-hydroxyecdysone 26-phosphate were 31 μg/g of ovaries from 4-day-old females and 25 μg/g and 17 μg/g of 48- to 64-hr-old and 72- to 88-hr-old eggs, respectively. The RP-HPLC of the conjugate fraction of 48- to 64-hr-old eggs showed an additional peak of radioactive material eluting about three min before the 26-hydroxyecdysone 26-phosphate. The quantity of this material increased in the 72-to 88-hr-old eggs, though it was not detected in the analyses of the conjugate fraction from ovaries. Additional peaks of radioactive material eluting before the 26-hydroxyecdysone 26-phosphate peak were observed in the chromatogram of the conjugates of 72- to 88-hr-old eggs. These radioactive materials need to be identified to determine the ultimate fate of ecdysteroids in the developing embryos of the tobacco hornworm. No radioactive free ecdysteroids were detected in either egg age group. © 1986 American Oil Chemists' Society.
Scientific Publication
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