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אסיף מאגר המחקר החקלאי
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Synthesis of bacteriophage phi e DNA in toluenized preparations of Bacillus subtilis infected cells
Year:
1974
Source of publication :
Israel Journal of Medical Sciences
Authors :
Lavi, Uri
;
.
Volume :
10
Co-Authors:
Marcus, M., Dept. Genet., Hebrew Univ., Jerusalem, Israel
Lavi, U., Dept. Genet., Hebrew Univ., Jerusalem, Israel
Nattenberg, A., Dept. Genet., Hebrew Univ., Jerusalem, Israel
Facilitators :
From page:
1500
To page:
1504
(
Total pages:
5
)
Abstract:
Phie is a Bacillus subtilis virulent phage which contains hydroxymethyluracil in its DNA instead of thymine. In phie infected cells, host DNA synthesis is arrested several minutes after infection and phage DNA synthesis begins. The results, based on DNA-DNA hybridization experiments, clearly show that the arrest of host DNA synthesis and the appearance of phage DNA synthesis are manifested faithfully in toluenized preparations of phie infected cells. These results also show that phie DNA synthesis is resistant to the reduced form of the drug 6-(p-hydroxyphenylazo)-uracil, which blocks B. subtilis DNA synthesis by inhibiting the activity of DNA polymerase III.
Note:
Related Files :
DNA Nucleotidyltransferases
dna synthesis
microorganism
Mutagens
phenols
Uracil
virulence
Show More
Related Content
More details
DOI :
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
18882
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:24
Scientific Publication
Synthesis of bacteriophage phi e DNA in toluenized preparations of Bacillus subtilis infected cells
10
Marcus, M., Dept. Genet., Hebrew Univ., Jerusalem, Israel
Lavi, U., Dept. Genet., Hebrew Univ., Jerusalem, Israel
Nattenberg, A., Dept. Genet., Hebrew Univ., Jerusalem, Israel
Synthesis of bacteriophage phi e DNA in toluenized preparations of Bacillus subtilis infected cells
Phie is a Bacillus subtilis virulent phage which contains hydroxymethyluracil in its DNA instead of thymine. In phie infected cells, host DNA synthesis is arrested several minutes after infection and phage DNA synthesis begins. The results, based on DNA-DNA hybridization experiments, clearly show that the arrest of host DNA synthesis and the appearance of phage DNA synthesis are manifested faithfully in toluenized preparations of phie infected cells. These results also show that phie DNA synthesis is resistant to the reduced form of the drug 6-(p-hydroxyphenylazo)-uracil, which blocks B. subtilis DNA synthesis by inhibiting the activity of DNA polymerase III.
Scientific Publication
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