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The role of aquaporins in ph-dependent germination of Rhizopus delemar spores
Year:
2016
Source of publication :
PLoS ONE
Authors :
Eshel, Dani
;
.
Lichter, Amnon
;
.
Teper-Bamnolker, Paula
;
.
Turgeman, Tidhar
;
.
Volume :
11
Co-Authors:
Turgeman, T., Department of Postharvest Sciences of Fresh Produce, Agricultural Research Organization (ARO), Volcani Center, Bet-Dagan, Israel, Department of Plant Sciences and Genetics in Agriculture, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel
Shatil-Cohen, A., Department of Plant Sciences and Genetics in Agriculture, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel
Moshelion, M., Department of Plant Sciences and Genetics in Agriculture, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel
Teper-Bamnolker, P., Department of Postharvest Sciences of Fresh Produce, Agricultural Research Organization (ARO), Volcani Center, Bet-Dagan, Israel
Skory, C.D., Renewable Product Technology Research Unit, NTL Center for Agricultural Utilization Research, Peoria, IL, United States
Lichter, A., Department of Postharvest Sciences of Fresh Produce, Agricultural Research Organization (ARO), Volcani Center, Bet-Dagan, Israel
Eshel, D., Department of Postharvest Sciences of Fresh Produce, Agricultural Research Organization (ARO), Volcani Center, Bet-Dagan, Israel
Facilitators :
From page:
0
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0
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Total pages:
1
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Abstract:
Rhizopus delemar and associated species attack a wide range of fruit and vegetables after harvest. Host nutrients and acidic pH are required for optimal germination of R. delemar, and we studied how this process is triggered. Glucose induced spore swelling in an acidic environment, expressed by an up to 3-fold increase in spore diameter, whereas spore diameter was smaller in a neutral environment.When suspended in an acidic environment, the spores started to float, indicating a change in their density. Treatment of the spores with HgCl2, an aquaporin blocker, prevented floating and inhibited spore swelling and germ-tube emergence, indicating the importance of water uptake at the early stages of germination. Two putative candidate aquaporin-encoding genes-RdAQP1 and RdAQP2-were identified in the R. delemar genome. Both presented the conserved NPA motif and six-transmembrane domain topology. Expressing RdAQP1 and RdAQP2 in Arabidopsis protoplasts increased the cells' osmotic water permeability coefficient (Pf) compared to controls, indicating their role as water channels. A decrease in R. delemar aquaporin activity with increasing external pH suggested pH regulation of these proteins. Substitution of two histidine (His) residues, positioned on two loops facing the outer side of the cell, with alanine eliminated the pH sensing resulting in similar Pf values under acidic and basic conditions. Since hydration is critical for spore switching from the resting to activate state, we suggest that pH regulation of the aquaporins can regulate the initial phase of R. delemar spore germination, followed by germ-tube elongation and host-tissue infection. This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.
Note:

Article number e0150543

Related Files :
Aquaporins
Fruits
postharvest injuries
postharvest physiology
Rhizopus
vegetables
Show More
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More details
DOI :
10.1371/journal.pone.0150543
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
18894
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:25
You may also be interested in
Scientific Publication
The role of aquaporins in ph-dependent germination of Rhizopus delemar spores
11
Turgeman, T., Department of Postharvest Sciences of Fresh Produce, Agricultural Research Organization (ARO), Volcani Center, Bet-Dagan, Israel, Department of Plant Sciences and Genetics in Agriculture, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel
Shatil-Cohen, A., Department of Plant Sciences and Genetics in Agriculture, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel
Moshelion, M., Department of Plant Sciences and Genetics in Agriculture, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel
Teper-Bamnolker, P., Department of Postharvest Sciences of Fresh Produce, Agricultural Research Organization (ARO), Volcani Center, Bet-Dagan, Israel
Skory, C.D., Renewable Product Technology Research Unit, NTL Center for Agricultural Utilization Research, Peoria, IL, United States
Lichter, A., Department of Postharvest Sciences of Fresh Produce, Agricultural Research Organization (ARO), Volcani Center, Bet-Dagan, Israel
Eshel, D., Department of Postharvest Sciences of Fresh Produce, Agricultural Research Organization (ARO), Volcani Center, Bet-Dagan, Israel
The role of aquaporins in ph-dependent germination of Rhizopus delemar spores
Rhizopus delemar and associated species attack a wide range of fruit and vegetables after harvest. Host nutrients and acidic pH are required for optimal germination of R. delemar, and we studied how this process is triggered. Glucose induced spore swelling in an acidic environment, expressed by an up to 3-fold increase in spore diameter, whereas spore diameter was smaller in a neutral environment.When suspended in an acidic environment, the spores started to float, indicating a change in their density. Treatment of the spores with HgCl2, an aquaporin blocker, prevented floating and inhibited spore swelling and germ-tube emergence, indicating the importance of water uptake at the early stages of germination. Two putative candidate aquaporin-encoding genes-RdAQP1 and RdAQP2-were identified in the R. delemar genome. Both presented the conserved NPA motif and six-transmembrane domain topology. Expressing RdAQP1 and RdAQP2 in Arabidopsis protoplasts increased the cells' osmotic water permeability coefficient (Pf) compared to controls, indicating their role as water channels. A decrease in R. delemar aquaporin activity with increasing external pH suggested pH regulation of these proteins. Substitution of two histidine (His) residues, positioned on two loops facing the outer side of the cell, with alanine eliminated the pH sensing resulting in similar Pf values under acidic and basic conditions. Since hydration is critical for spore switching from the resting to activate state, we suggest that pH regulation of the aquaporins can regulate the initial phase of R. delemar spore germination, followed by germ-tube elongation and host-tissue infection. This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.

Article number e0150543

Scientific Publication
You may also be interested in